Yoshida K, Narita M, Fujinaga K
Department of Molecular Biology, Cancer Research Institute, Sapporo Medical College, Japan.
Nucleic Acids Res. 1989 Dec 11;17(23):10015-34. doi: 10.1093/nar/17.23.10015.
Twenty one binding sites of HeLa cell nuclear proteins were identified on the upstream region of adenovirus type 5 E1A gene using DNase I footprint assay. The proximal promoter region contained five binding sites that overlapped the cap site, TATA box, TATA-like sequence, CCAAT box, and -100 region relative to the E1A cap site(+1). The -190 region was a potential site for octamer-motif binding proteins, such as NFIII and OBP100. An upstream copy of the E1A enhancer element 1 was the site for a factor (E1A-F) with the binding specificity of XGGAYGT (X = A, C; Y = A, T). E1A-F factor also bound to three other sites, one of which coincided with the distal E1A enhancer element. The distal element also contained a potential site for ATF factor. The adenovirus minimal origin of DNA replication competed for DNA-protein complex formation on the CCAAT and TATA box region and the -190 region, suggesting that these regions interacted with a common or related factor.
使用DNA酶I足迹分析法在5型腺病毒E1A基因上游区域鉴定出21个HeLa细胞核蛋白结合位点。近端启动子区域包含5个结合位点，它们与帽位点、TATA盒、类TATA序列、CCAAT盒以及相对于E1A帽位点（+1）的-100区域重叠。-190区域是八聚体基序结合蛋白（如NFIII和OBP100）的潜在结合位点。E1A增强子元件1的上游拷贝是具有XGGAYGT（X = A、C；Y = A、T）结合特异性的因子（E1A-F）的结合位点。E1A-F因子还与其他三个位点结合，其中一个与远端E1A增强子元件重合。远端元件还包含一个ATF因子的潜在结合位点。腺病毒最小DNA复制起点在CCAAT和TATA盒区域以及-190区域竞争DNA-蛋白质复合物的形成，这表明这些区域与一个共同或相关因子相互作用。
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