Lu Zhen Qi, Sinha Ankit, Sharma Parveen, Kislinger Thomas, Gramolini Anthony O
Department of Physiology, ‡Medical Biophysics, Faculty of Medicine, and §Heart and Stroke/Richard Lewar Centre of Excellent for Cardiovascular Research, University of Toronto , Toronto, Ontario, Canada.
J Proteome Res. 2014 Dec 5;13(12):5869-78. doi: 10.1021/pr5007685. Epub 2014 Oct 31.
In this study we carried out a mass spectrometry-based proteome analysis of human fetal atria and ventricles. Heart protein lysates were analyzed on the Q-Exactive mass spectrometer in biological triplicates. Protein identification using MaxQuant yielded a total of 2754 atrial protein groups (91%) and 2825 ventricular protein groups (83%) in at least 2 of the 3 runs with ≥ 2 unique peptides. Statistical analyses using fold-enrichment (>2) and p-values (≤ 0.05) selected chamber-enriched atrial (134) and ventricular (81) protein groups. Several previously characterized cardiac chamber-enriched proteins were identified in this study including atrial isoform of myosin light chain 2 (MYL7), atrial natriuretic peptide (NPPA), connexin 40 (GJA5), and peptidylglycine alpha-amidating monooxygenase (PAM) for atria, and ventricular isoforms of myosin light chains (MYL2 and MYL3), myosin heavy chain 7 (MYH7), and connexin 43 (GJA1) for ventricle. Our data was compared to in-house generated and publicly available human microarrays, several human cardiac proteomes, and phenotype ontology databases.
在本研究中，我们对人类胎儿的心房和心室进行了基于质谱的蛋白质组分析。心脏蛋白质裂解物在Q-Exactive质谱仪上进行了三次生物学重复分析。使用MaxQuant进行蛋白质鉴定，在至少2次运行中，≥2条独特肽段的情况下，共鉴定出2754个心房蛋白组（91%）和2825个心室蛋白组（83%）。使用富集倍数（>2）和p值（≤0.05）进行统计分析，筛选出心房富集的134个蛋白组和心室富集的81个蛋白组。本研究鉴定出了几种先前已表征的心脏腔室富集蛋白，包括心房肌球蛋白轻链2（MYL7）、心房利钠肽（NPPA）、连接蛋白40（GJA5）和肽基甘氨酸α-酰胺化单加氧酶（PAM），以及心室肌球蛋白轻链（MYL2和MYL3）、肌球蛋白重链7（MYH7）和连接蛋白43（GJA1）的心室异构体。我们的数据与内部生成的和公开可用的人类微阵列、几个人类心脏蛋白质组以及表型本体数据库进行了比较。
