Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz, Lodz, Poland.
Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz, Lodz, Poland.
Adv Med Sci. 2014 Sep;59(2):178-82. doi: 10.1016/j.advms.2014.02.005. Epub 2014 Jun 9.
Numerous studies have suggested that grape seed extract (GSE) confers vascular protection due to the direct effect of its polyphenol content on endothelial cells. The aim of the study was to determine whether GSE confers vascular protection through the direct effect of its polyphenol content on endothelial cells.
MATERIAL/METHODS: After incubation with GSE-treated human umbilical vein endothelial cells (HUVECs), blood platelet reactivity was evaluated with regard to the expression of CD62P and the activated form of GPIIbIIIa in ADP-stimulated platelets.
Lower concentrations of GSE were found to enhance the antiplatelet action of HUVECs: 1 μg/ml GSE reduced platelet reactivity by about 10%. While platelet reactivity was not altered by HUVECs incubated with higher concentrations of GSE, HUVEC proliferation was significantly reduced by GSE of up to 10 μg gallic acid equivalent/ml.
The results of the study show that low doses of GSE potentiate the inhibitory action of HUVECs on platelet reactivity, which may account, at least partially, for the protective effects of grape products against cardiovascular diseases. In contrast, high concentrations of GSE significantly impair endothelial cell proliferation in vitro.
大量研究表明，葡萄籽提取物（GSE）通过其多酚含量对内皮细胞的直接作用赋予血管保护作用。本研究旨在确定 GSE 是否通过其多酚含量对内皮细胞的直接作用赋予血管保护作用。
材料/方法：用人脐静脉内皮细胞（HUVEC）孵育 GSE 处理后，评估 ADP 刺激血小板中 CD62P 和 GPIIbIIIa 活化形式的表达，以评估血小板反应性。
发现较低浓度的 GSE 增强了 HUVEC 的抗血小板作用：1μg/ml 的 GSE 使血小板反应性降低了约 10%。虽然用较高浓度的 GSE 孵育 HUVEC 不会改变血小板反应性，但高达 10μg 没食子酸当量/ml 的 GSE 可显著减少 HUVEC 的增殖。
研究结果表明，低剂量的 GSE 增强了 HUVEC 对血小板反应性的抑制作用，这至少部分解释了葡萄产品对心血管疾病的保护作用。相比之下，高浓度的 GSE 会显著损害体外内皮细胞的增殖。
