转录因子HoxB9的高表达预示肺腺癌患者预后不良。

High expression of transcriptional factor HoxB9 predicts poor prognosis in patients with lung adenocarcinoma.

作者信息

Zhan Jun, Wang Peng, Niu Miaomiao, Wang Yunling, Zhu Xiang, Guo Yongqing, Zhang Hongquan

机构信息

Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, and State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing, China.

Department of Anatomy, Histology and Embryology, Laboratory of Molecular Cell Biology and Tumor Biology, Beijing, China.

出版信息

Histopathology. 2015 Jun;66(7):955-65. doi: 10.1111/his.12585. Epub 2015 Feb 8.

DOI:10.1111/his.12585

PMID:25324169

Abstract

AIMS

HoxB9, as a Hox family member, is known to play important roles in embryonic development. Recent studies showed that HoxB9 is engaged in cancer progression. However, the role of Hoxb9 in lung adenocarcinoma is unknown. The purpose of this study is to investigate the expression and prognostic value of HoxB9 in patients with lung adenocarcinoma.

METHODS AND RESULTS

The localization and expression of HoxB9 in lung adenocarcinoma were examined by immunohistochemistry. The correlation between HoxB9 expression levels with patient survival was assessed by Kaplan-Meier analysis. The epithelial-mesenchymal transition (EMT) markers and migratory ability were evaluated in HoxB9 up- and down-regulated H1299 lung adenocarcinoma cells. HoxB9 was found to be localized predominantly in the cell nuclei and expressed in 21.3% of lung adenocarcinomas. A significant increase in HoxB9 intensity in the high stage of lung adenocarcinoma was observed (P < 0.01). Increased expression of HoxB9 was related to T classification, more lymph node metastasis and a shorter patient overall survival (P < 0.05). However, the expression level of HoxB9 was not correlated with age and gender. Functionally, HoxB9 up-regulated EMT-related molecules and promoted cell migration in H1299 cells.

CONCLUSION

High expression of HoxB9 is a prognostic marker for lung adenocarcinoma patients.

摘要

目的

HoxB9作为Hox家族成员，已知在胚胎发育中发挥重要作用。最近的研究表明，HoxB9参与癌症进展。然而，Hoxb9在肺腺癌中的作用尚不清楚。本研究的目的是探讨HoxB9在肺腺癌患者中的表达及预后价值。

方法与结果

采用免疫组织化学法检测HoxB9在肺腺癌中的定位和表达。通过Kaplan-Meier分析评估HoxB9表达水平与患者生存率之间的相关性。在HoxB9上调和下调的H1299肺腺癌细胞中评估上皮-间质转化（EMT）标志物和迁移能力。发现HoxB9主要定位于细胞核，在21.3%的肺腺癌中表达。观察到肺腺癌高分期中HoxB9强度显著增加（P < 0.01）。HoxB9表达增加与T分类、更多的淋巴结转移和患者总生存期缩短相关（P < 0.05）。然而，HoxB9的表达水平与年龄和性别无关。在功能上，HoxB9上调H1299细胞中与EMT相关的分子并促进细胞迁移。