Ni Jin, Romero-Weaver Ana L, Kennedy Ann R
Department of Radiation Oncology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania 19104, USA; ; Current Address: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China.
Department of Radiation Oncology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania 19104, USA;
Int J Biomed Sci. 2014 Sep;10(3):182-90.
Si-Wu-Tang (SWT) is a decoction consisting of a mixture of ingredients of Rehmanniae Radix, Angelica Radix, Chuanxiong Rhizoma and Paeoniae Radix. As a traditional Chinese herbal decoction, SWT has been widely used for the treatment of diseases characterized as blood and/or energy deficit. The present study was performed to evaluate the effects of SWT on the different populations of circulating white blood cells (WBCs) and gastrointestinal changes in γ-ray irradiated mice. Female mice were treated daily with orally administered SWT seven days before irradiation, until one day before irradiation or until one day before sample collection. WBC counts were determined from peripheral blood samples taken from the mice at different times post-irradiation. Hematoxylin and eosin (H&E) staining, as well as immunohistochemical analysis of fibrinogen, were utilized to evaluate the effects of SWT in the intestines of mice after radiation exposure. The results of the present studies demonstrate that SWT has protective effects against radiation damage to circulating WBCs, specifically to lymphocytes, and to the gastrointestinal tract of the irradiated animals.
四物汤（SWT）是一种由熟地黄、当归、川芎和白芍混合而成的汤剂。作为一种传统的中草药汤剂，四物汤已被广泛用于治疗以血虚和/或气虚为特征的疾病。本研究旨在评估四物汤对γ射线照射小鼠循环白细胞（WBC）不同亚群及胃肠道变化的影响。雌性小鼠在照射前7天每天口服四物汤，持续至照射前1天或样本采集前1天。在照射后不同时间从小鼠采集外周血样本，测定白细胞计数。利用苏木精和伊红（H&E）染色以及纤维蛋白原的免疫组织化学分析，评估四物汤对辐射暴露后小鼠肠道的影响。本研究结果表明，四物汤对辐射引起的循环白细胞，特别是淋巴细胞以及受照射动物胃肠道的损伤具有保护作用。
