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人胎盘中脂皮质素-1的二聚体形式。

A dimeric form of lipocortin-1 in human placenta.

作者信息

Pepinsky R B, Sinclair L K, Chow E P, O'Brine-Greco B

机构信息

Biogen Inc., Cambridge, MA 02142.

出版信息

Biochem J. 1989 Oct 1;263(1):97-103. doi: 10.1042/bj2630097.

DOI:10.1042/bj2630097
PMID:2532504
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1133395/
Abstract

We have characterized a 68 kDa lipocortin from human placenta that was identified as a covalently linked homodimer of lipocortin-1 by peptide mapping and sequence analysis. The site of cross-linking was localized within the 3 kDa N-terminal tail region, an exposed domain that contains the phosphorylation sites for protein tyrosine kinase and protein kinase C and is sensitive to proteolysis. Sequence analysis of the corresponding peptide revealed that glutamine-18 was modified, suggesting that the cross-link may be generated by a transglutaminase. By incubating lipocortin-1 with placental membranes and with labelled glycine ethyl ester we observed a Ca2+-dependent labelling of lipocortin-1 within the tail region, supporting this notion. Like lipocortin-1, the dimer inhibits phospholipase Ad2 activity, is a substrate for the epidermal-growth-factor (EGF) receptor/kinase, and display Ca2+-dependent binding to phosphatidylserine-containing vesicles. In preparations from human placenta the dimer is particularly abundant, accounting for approx. 20% of the lipocortin-1.

摘要

我们已对来自人胎盘的一种68 kDa脂皮质素进行了表征，通过肽图谱分析和序列分析确定其为脂皮质素-1的共价连接同源二聚体。交联位点定位于3 kDa的N末端尾部区域内，该区域是一个暴露的结构域，包含蛋白酪氨酸激酶和蛋白激酶C的磷酸化位点，并且对蛋白水解敏感。对相应肽段的序列分析表明谷氨酰胺-18发生了修饰，提示交联可能由转谷氨酰胺酶产生。通过将脂皮质素-1与胎盘膜以及标记的甘氨酸乙酯一起孵育，我们观察到脂皮质素-1在尾部区域有Ca2+依赖性标记，支持了这一观点。与脂皮质素-1一样，该二聚体抑制磷脂酶Ad2活性，是表皮生长因子（EGF）受体/激酶的底物，并表现出对含磷脂酰丝氨酸囊泡的Ca2+依赖性结合。在人胎盘的制剂中，该二聚体特别丰富，约占脂皮质素-1的20%。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da25/1133395/6fced2376fa7/biochemj00198-0106-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da25/1133395/c429826b635a/biochemj00198-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da25/1133395/73f6127ed668/biochemj00198-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da25/1133395/c4c42cf987d2/biochemj00198-0103-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da25/1133395/a81034144020/biochemj00198-0105-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da25/1133395/6fced2376fa7/biochemj00198-0106-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da25/1133395/c429826b635a/biochemj00198-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da25/1133395/73f6127ed668/biochemj00198-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da25/1133395/c4c42cf987d2/biochemj00198-0103-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da25/1133395/a81034144020/biochemj00198-0105-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da25/1133395/6fced2376fa7/biochemj00198-0106-a.jpg

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本文引用的文献

1
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J Biol Chem. 1981 Aug 10;256(15):7990-7.
2
Isolation of a calcium-dependent 35-kilodalton substrate for the epidermal growth factor receptor/kinase from A-431 cells.从A-431细胞中分离表皮生长因子受体/激酶的一种钙依赖性35千道尔顿底物。
J Biol Chem. 1984 Feb 25;259(4):2636-45.
3
Localization of lipid-protein and protein-protein interactions within the murine retrovirus gag precursor by a novel peptide-mapping technique.利用一种新型肽图谱技术对小鼠逆转录病毒gag前体中的脂蛋白和蛋白质-蛋白质相互作用进行定位。
Br J Pharmacol. 2008 Sep;155(2):152-69. doi: 10.1038/bjp.2008.252. Epub 2008 Jul 21.
4
Nuclear annexin II negatively regulates growth of LNCaP cells and substitution of ser 11 and 25 to glu prevents nucleo-cytoplasmic shuttling of annexin II.核膜联蛋白II负向调节LNCaP细胞的生长，将丝氨酸11和25替换为谷氨酸可阻止膜联蛋白II在细胞核与细胞质之间穿梭。
BMC Biochem. 2003 Sep 9;4:10. doi: 10.1186/1471-2091-4-10.
5
Annexin 1 (lipocortin 1) mimics inhibitory effects of glucocorticoids on testosterone secretion and enhances effects of interleukin-1beta.膜联蛋白1（脂皮质蛋白1）模拟糖皮质激素对睾酮分泌的抑制作用，并增强白细胞介素-1β的作用。
Endocrine. 2002 Jun;18(1):33-9. doi: 10.1385/ENDO:18:1:33.
6
Caloric restriction as a mechanism mediating resistance to environmental disease.热量限制作为一种介导对环境疾病抵抗力的机制。
Environ Health Perspect. 1998 Feb;106 Suppl 1(Suppl 1):313-24. doi: 10.1289/ehp.98106s1313.
7
The role of lipocortin-1 in dexamethasone-induced suppression of PGE2 and TNF alpha release from human peripheral blood mononuclear cells.脂皮质素-1在地塞米松诱导抑制人外周血单核细胞释放前列腺素E2和肿瘤坏死因子α中的作用。
Br J Pharmacol. 1996 Apr;117(7):1449-56. doi: 10.1111/j.1476-5381.1996.tb15305.x.
8
Evidence for specific annexin I-binding proteins on human monocytes.人类单核细胞上特异性膜联蛋白I结合蛋白的证据。
Biochem J. 1996 Jun 1;316 ( Pt 2)(Pt 2):593-7. doi: 10.1042/bj3160593.
9
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10
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4
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5
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6
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Nature. 1986;320(6063):573. doi: 10.1038/320573a0.
7
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FEBS Lett. 1987 Nov 2;223(2):267-72. doi: 10.1016/0014-5793(87)80302-2.
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FEBS Lett. 1987 Apr 6;214(1):181-6. doi: 10.1016/0014-5793(87)80038-8.
9
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Biochim Biophys Acta. 1987 Dec 18;916(3):288-97.
10
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EMBO J. 1988 Jan;7(1):21-7. doi: 10.1002/j.1460-2075.1988.tb02779.x.