Grisenti Michela, Vázquez Ana, Herrero Laura, Cuevas Laureano, Perez-Pastrana Esperanza, Arnoldi Daniele, Rosà Roberto, Capelli Gioia, Tenorio Antonio, Sánchez-Seco Maria Paz, Rizzoli Annapaola
Department of Veterinary Sciences, University of Torino, largo Paolo Braccini 2, 10095 Grugliasco, Torino, Italy.
Department of Biodiversity and Molecular Ecology, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 San Michele all'Adige, Trento, Italy.
J Gen Virol. 2015 Feb;96(Pt 2):420-430. doi: 10.1099/vir.0.069625-0. Epub 2014 Oct 17.
The pattern of flavivirus infection in mosquitoes belonging to the genera Aedes and Culex collected in two regions of north-eastern Italy (Trentino and Veneto) was assessed. Mosquitoes were collected during 2012 and screened for flaviviruses using a generic reverse transcription-nested-PCR targeted on a region of the non-structural NS5 gene. The phylogenetic analysis was performed on a fragment of ~1000 bp. Virus isolation was attempted in C6/36 insect cell lines and the infected cell cultures were studied by electron microscopy. We detected a wide distribution of Aedes flavivirus (AeFV) in Aedes albopictus, with higher infection prevalence in Trentino than in Veneto. In Culex pipiens collected in Veneto, we detected a new sequence of an insect-specific flavivirus and one of Usutu virus. Interestingly, we detected AeFV in C. pipiens, for the first time to our knowledge, in both regions. Viral isolation in cell culture was successful for AeFV. AeFV sequences found in Veneto showed a high percentage of similarity to those detected in Trentino and to those previously reported in other areas of northern Italy. Co-infections with different flaviviruses were not detected.
对在意大利东北部两个地区（特伦蒂诺和威尼托）采集的伊蚊属和库蚊属蚊子中的黄病毒感染模式进行了评估。在2012年期间收集蚊子，并使用针对非结构NS5基因区域的通用逆转录巢式PCR对黄病毒进行筛查。对约1000 bp的片段进行了系统发育分析。尝试在C6/36昆虫细胞系中进行病毒分离，并通过电子显微镜对感染的细胞培养物进行研究。我们在白纹伊蚊中检测到广泛分布的伊蚊黄病毒（AeFV），特伦蒂诺的感染率高于威尼托。在威尼托采集的致倦库蚊中，我们检测到一种昆虫特异性黄病毒的新序列和乌苏图病毒的一个序列。有趣的是，据我们所知，在两个地区的致倦库蚊中均首次检测到AeFV。AeFV在细胞培养中的病毒分离成功。在威尼托发现的AeFV序列与在特伦蒂诺检测到的以及之前在意大利北部其他地区报道的序列具有高度相似性。未检测到不同黄病毒的共同感染。
