Water Desalination and Reuse Research Center, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia.
Appl Microbiol Biotechnol. 2015 Mar;99(5):2361-70. doi: 10.1007/s00253-014-6134-4. Epub 2014 Oct 19.
In this study, 16S rRNA gene pyrosequencing was applied in order to provide a better insight on the diversity and dynamics of total, dominant, and rare bacterial taxa in replicate lab-scale sequencing batch reactors (SBRs) operated at different solids retention time (SRT). Rank-abundance curves showed few dominant operational taxonomic units (OTUs) and a long tail of rare OTUs in all reactors. Results revealed that there was no detectable effect of SRT (2 vs. 10 days) on Shannon diversity index and OTU richness of both dominant and rare taxa. Nonmetric multidimensional scaling analysis showed that the total, dominant, and rare bacterial taxa were highly dynamic during the entire period of stable reactor performance. Also, the rare taxa were more dynamic than the dominant taxa despite expected low invasion rates because of the use of sterile synthetic media.
在这项研究中，应用 16S rRNA 基因焦磷酸测序技术，以便更好地了解在不同固体停留时间（SRT）下运行的重复实验室规模序批式反应器（SBR）中总、优势和稀有细菌类群的多样性和动态变化。等级丰度曲线显示，所有反应器中都有少数优势操作分类单元（OTUs）和大量稀有 OTUs。结果表明，SRT（2 天与 10 天）对优势和稀有类群的香农多样性指数和 OTU 丰富度均无明显影响。非度量多维尺度分析表明，在整个稳定反应器性能期间，总细菌、优势细菌和稀有细菌类群具有高度动态性。此外，尽管由于使用无菌合成培养基，稀有类群的入侵率预计较低，但它们比优势类群更具动态性。
