Yang Guohua, Yang Lei, Zhuang Yun, Qian Xifeng, Shen Yunfeng
a Department of Hematology, Wu Xi People's Hospital , Nanjing Medical University , Wuxi , People's Republic of China.
J Recept Signal Transduct Res. 2016;36(1):6-13. doi: 10.3109/10799893.2014.970275. Epub 2014 Oct 20.
In this study, we investigated the anti-tumor activity both in vitro and in vivo of a polysaccharide obtained from Ganoderma lucidum on HL-60 acute myeloid leukemia cells, and focused on its targeting effect on mitogen-activated protein kinase (MAPK) pathways. It was found by the methods such as western blot and flow cytometry (FCM), that G. lucidum polysaccharide (GLP) blocked the extracellular signal-regulated kinase/MAPK signaling pathway, simultaneously activated p38 and JNK MAPK pathways, and therefore regulated their downstream genes and proteins, including p53, c-myc, c-fos, c-jun, Bcl-2, Bax, cleaved caspase-3 and cyclin D1. As a result, cycle arrest and apoptosis of HL-60 cells were induced. Therefore, GLP exerted anti-tumor activity via MAPK pathways in HL-60 acute leukemia cells.
在本研究中，我们调查了从灵芝中获得的一种多糖对HL-60急性髓系白血病细胞的体外和体内抗肿瘤活性，并重点研究了其对丝裂原活化蛋白激酶（MAPK）通路的靶向作用。通过蛋白质免疫印迹和流式细胞术（FCM）等方法发现，灵芝多糖（GLP）阻断细胞外信号调节激酶/MAPK信号通路，同时激活p38和JNK MAPK通路，从而调节其下游基因和蛋白质，包括p53、c-myc、c-fos、c-jun、Bcl-2、Bax、裂解的半胱天冬酶-3和细胞周期蛋白D1。结果，诱导了HL-60细胞的细胞周期停滞和凋亡。因此，GLP通过MAPK通路在HL-60急性白血病细胞中发挥抗肿瘤活性。
