Wang Xiaojiang, Zheng Xiongwei, Lin Xiandong, Shi Yi, He Yinzhu, Chen Gang
Department of Molecular Pathology, Fujian Provincial Cancer Hospital, Fuzhou 350014, China.
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Zhonghua Bing Li Xue Za Zhi. 2014 Jul;43(7):447-50.
To investigate the methylation status of Runx3 promoter and Runx3 expression in breast lesion tissues.
One hundred and fourteen breast lesions, including 35 cases of fibroadenoma, 39 cases of intraductal carcinoma, 40 cases of invasive ductal carcinoma, and 33 cases of normal breast tissue from Fabruary 2010 to August 2012 were included in this study. Runx3 protein expression was assessed by immunohistochemical SP method; whereas methylation of Runx3 promoter was assessed by high resolution melting (HRM) analysis.
Runx3 protein was mainly expressed in the cytoplasm of ductal epithelial cells. The expression rates of Runx3 in normal breast tissue, fibroadenoma, ductal carcinoma in situ, invasive ductal carcinoma were 87.9% (29/33), 85.7% (30/35), 53.8% (21/39), and 40.0% (16/40) respectively. The methylation rates of Runx3 promoter were 12.1% (4/33), 20.0% (7/35), 46.2% (18/39), and 57.5% (23/40), respectively. Correlation analysis between promoter methylation and protein expression of Runx3 in different breast tissue showed the r value in normal breast tissue, fibroadenoma, ductal carcinoma in situ and invasive ductal carcinoma was -0.431 (P = 0.012), -0.408 (P = 0.015), -0.589 (P = 0.000) and -0.743 (P = 0.000) respectively.
Runx3 protein expression shows a downward trend in ductal carcinoma in situ and invasive ductal carcinoma, meanwhile its promoter methylation increases significantly. The methylation of Runx3 promoter may be one of the important factors in the occurrence and development of breast cancer.
探讨Runx3启动子的甲基化状态及Runx3在乳腺病变组织中的表达情况。
本研究纳入了2010年2月至2012年8月期间的114例乳腺病变，包括35例纤维腺瘤、39例导管内癌、40例浸润性导管癌以及33例正常乳腺组织。采用免疫组织化学SP法评估Runx3蛋白表达；而通过高分辨率熔解（HRM）分析评估Runx3启动子的甲基化情况。
Runx3蛋白主要表达于导管上皮细胞的细胞质中。Runx3在正常乳腺组织、纤维腺瘤、导管原位癌、浸润性导管癌中的表达率分别为87.9%（29/33）、85.7%（30/35）、53.8%（21/39）和40.0%（16/40）。Runx3启动子的甲基化率分别为12.1%（4/33）、20.0%（7/35）、46.2%（18/39）和57.5%（23/40）。不同乳腺组织中Runx3启动子甲基化与蛋白表达的相关性分析显示，正常乳腺组织、纤维腺瘤、导管原位癌和浸润性导管癌中的r值分别为-0.43 (P = 0.012)、-0.408 (P = 0.015)、-0.589 (P = 0.000)和-0.743 (P = 0.000)。
Runx3蛋白表达在导管原位癌和浸润性导管癌中呈下降趋势，同时其启动子甲基化显著增加。Runx3启动子的甲基化可能是乳腺癌发生发展的重要因素之一。
