Institute Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, 250100 China.
Institute Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, 250100 China ; Institute Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, No. 198, Gongyebei Road, Jinan, 250100 China.
J Food Sci Technol. 2014 Oct;51(10):2851-6. doi: 10.1007/s13197-012-0801-1. Epub 2012 Aug 24.
Soybean protein was taken as a model protein to investigate two aspects of the protein extraction by sodium bis(2-ethylhexyl) sulfosuccinate (AOT) reverse micelles: (1) the forward protein extraction from the solid state, and the effect of pH, AOT concentration, alcohol and water content (W0) on the transfer efficiency; (2) the back-transfer, the capability of the protein to be recovered from the micellar solution. The experimental results led to the conclusion that the highest forward extraction efficiency of soybean protein was reached at AOT concentration 180 mmol l(-1), aqueous pH 7.0, KCl concentration 0.05 mol l(-1), 0.5 % (v/v) alcohol, W0 18. Under these conditions, the forward extraction efficiency of soybean protein achieved 70.1 %. It was noted that the percentage of protein back extraction depended on the salt concentration and pH value. Around 92 % of protein recovery was obtained after back extraction.
以大豆蛋白为模型蛋白，研究了双（2-乙基己基）磺基琥珀酸钠（AOT）反胶束萃取蛋白质的两个方面：（1）从固态进行正向蛋白质萃取，以及 pH 值、AOT 浓度、醇和水含量（W0）对传递效率的影响；（2）反向转移，即从胶束溶液中回收蛋白质的能力。实验结果表明，在 AOT 浓度 180mmol·l^(-1)、水相 pH 值 7.0、KCl 浓度 0.05mol·l^(-1)、0.5%（v/v）醇、W0 为 18 的条件下，大豆蛋白的正向萃取效率达到 70.1%。值得注意的是，蛋白质的反向萃取百分比取决于盐浓度和 pH 值。经过反向萃取后，可获得约 92%的蛋白质回收率。
