不同喂养时间表对人胚胎干细胞和诱导多能干细胞存活及神经分化的影响。
Effect of different feeding schedules on the survival and neural differentiation of human embryonic and induced pluripotent stem cells.
作者信息
Jensen Matthew B, Jager Lindsey D, Cohen Laura K, Kwok Susanna S, Kwon Jin M, Hall Crystal A
机构信息
University of Wisconsin Department of Neurology.
出版信息
J Neurol Sci Turk. 2014;31(2):226-231.
Neural culture of human pluripotent stem cells is useful for neuroscience research, but the optimal feeding schedule for these in vitro systems is unclear. We evaluated the survival and neural differentiation profiles of human embryonic and induced pluripotent stem cells cultured with medium exchange schedules of five, six, or seven days weekly through two months of differentiation. No significant differences were seen in cell numbers or neural differentiation markers through this culture interval with either human pluripotent cell type. We conclude that there is unlikely to be an advantage of feeding more than five days weekly for this culture system.
人类多能干细胞的神经培养对神经科学研究很有用，但这些体外系统的最佳换液时间表尚不清楚。我们评估了人类胚胎干细胞和诱导多能干细胞在每周进行五天、六天或七天换液时间表培养两个月后的存活情况和神经分化情况。在这个培养期间，两种人类多能干细胞类型在细胞数量或神经分化标志物方面均未观察到显著差异。我们得出结论，对于这个培养系统而言，每周换液超过五天不太可能有优势。
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