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利用线粒体DNA细胞色素氧化酶亚基I(mtDNA COI)序列探究印度古吉拉特邦海岸几种新记录潮间带群体海葵的系统发育关系。

Probing the phylogenetic relationships of a few newly recorded intertidal zoanthids of Gujarat coast (India) with mtDNA COI sequences.

作者信息

Joseph Sneha, Poriya Paresh, Kundu Rahul

机构信息

a Department of Bioscience , Saurashtra University , Rajkot , Gujarat , India.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):3858-3864. doi: 10.3109/19401736.2014.971239. Epub 2014 Oct 20.

Abstract

The present study reports the phylogenetic relationship of six zoanthid species belonging to three genera, Isaurus, Palythoa, and Zoanthus identified using systematic computational analysis of mtDNA gene sequences. All six species are first recorded from the coasts of Kathiawar Peninsula, India. Genus: Isaurus is represented by Isaurus tuberculatus, genus Zoanthus is represented by Zoanthus kuroshio and Zoanthus sansibaricus, while genus Palythoa is represented by Palythoa tuberculosa, P. sp. JVK-2006 and Palythoa heliodiscus. Results of the present study revealed that among the various species observed along the coastline, a minimum of 99% sequence divergence and a maximum of 96% sequence divergence were seen. An interspecific divergence of 1-4% and negligible intraspecific divergence was observed. These results not only highlighted the efficiency of the COI gene region in species identification but also demonstrated the genetic variability of zoanthids along the Saurashtra coastline of the west coast of India.

摘要

本研究报告了通过对线粒体DNA基因序列进行系统计算分析鉴定出的属于三个属(伊索海葵属、沙群海葵属和花群海葵属)的六种群体海葵的系统发育关系。所有六个物种均首次记录于印度卡提阿瓦半岛海岸。伊索海葵属由瘤状伊索海葵代表,花群海葵属由黑潮花群海葵和桑给巴尔花群海葵代表,而沙群海葵属由瘤状沙群海葵、P. sp. JVK - 2006和日光盘沙群海葵代表。本研究结果显示,在所观察到的沿海岸线的各种物种中,序列差异最小值为99%,最大值为96%。观察到种间差异为1 - 4%,种内差异可忽略不计。这些结果不仅突出了细胞色素氧化酶亚基I(COI)基因区域在物种鉴定中的效率,还证明了印度西海岸索拉什特拉海岸线群体海葵的遗传变异性。

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