利用离子激流个人基因组测序仪通过高通量测序获得的入侵性 Ponticola kessleri（ Ponticola kessleri 为里海-黑海的一种虾虎鱼）的完整线粒体基因组。
The complete mitochondrial genome of the invasive Ponto-Caspian goby Ponticola kessleri obtained from high-throughput sequencing using the Ion Torrent Personal Genome Machine.
作者信息
Kalchhauser Irene, Kutschera Verena E, Burkhardt-Holm Patricia
机构信息
a Department of Environmental Science , Programm MGU, University of Basel , Basel , Switzerland and.
b Biodiversität und Klima Forschungszentrum , Senckenberganlage 25 , Frankfurt am Main , Germany.
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1887-9. doi: 10.3109/19401736.2014.971272. Epub 2014 Oct 20.
We report the first complete mitochondrial genome (mitogenome) of an invasive Ponto-Caspian goby, Ponticola kessleri (bighead goby, Günther 1891). Ion Torrent PGM sequencing of total DNA from two individuals yielded a contig of 16,971 bp, with overlapping ends located in the repetitive control region, which was validated using Sanger sequencing. The final mitogenome of Ponticola kessleri has a size of 16,890 bp and contains the expected gene configuration of 13 protein-coding genes, 2 rRNA genes and 22 tRNA genes. In a comparison with complete mitogenomes from other goby species, we identified a translocation of tRNA-Glu in the mitogenome of P. kessleri. Rearrangements are unique and rare events, and can thus provide phylogenetic information.
我们报告了入侵性 Pontic-Caspian 虾虎鱼（Ponticola kessleri，即大头虾虎鱼，冈瑟，1891 年）的首个完整线粒体基因组（线粒体基因组）。对两个个体的总 DNA 进行 Ion Torrent PGM 测序，得到了一个 16,971 bp 的重叠群，其重叠末端位于重复控制区，该重叠群通过桑格测序进行了验证。Ponticola kessleri 的最终线粒体基因组大小为 16,890 bp，包含 13 个蛋白质编码基因、2 个 rRNA 基因和 22 个 tRNA 基因的预期基因配置。在与其他虾虎鱼物种的完整线粒体基因组进行比较时，我们在 P. kessleri 的线粒体基因组中发现了 tRNA-Glu 的易位。重排是独特且罕见的事件，因此可以提供系统发育信息。
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