Kumar Girish, Kocour Martin, Kunal Swaraj Priyaranjan
a South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice , Vodnany , Czech Republic and.
b Biological Oceanography Division , National Institute of Oceanography , Panaji , India.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1976-80. doi: 10.3109/19401736.2014.971313. Epub 2014 Oct 20.
In order to assess the DNA sequence variation and phylogenetic relationship among five tuna species (Auxis thazard, Euthynnus affinis, Katsuwonus pelamis, Thunnus tonggol, and T. albacares) out of all four tuna genera, partial sequences of the mitochondrial DNA (mtDNA) D-loop region were analyzed. The estimate of intra-specific sequence variation in studied species was low, ranging from 0.027 to 0.080 [Kimura's two parameter distance (K2P)], whereas values of inter-specific variation ranged from 0.049 to 0.491. The longtail tuna (T. tonggol) and yellowfin tuna (T. albacares) were found to share a close relationship (K2P = 0.049) while skipjack tuna (K. pelamis) was most divergent studied species. Phylogenetic analysis using Maximum-Likelihood (ML) and Neighbor-Joining (NJ) methods supported the monophyletic origin of Thunnus species. Similarly, phylogeny of Auxis and Euthynnus species substantiate the monophyly. However, results showed a distinct origin of K. pelamis from genus Thunnus as well as Auxis and Euthynnus. Thus, the mtDNA D-loop region sequence data supports the polyphyletic origin of tuna species.
为了评估四个金枪鱼属中五个金枪鱼物种（扁舵鲣、羽鳃鲔、鲣、长腰金枪鱼和黄鳍金枪鱼）之间的DNA序列变异和系统发育关系，对线粒体DNA（mtDNA）D环区域的部分序列进行了分析。研究物种的种内序列变异估计值较低，范围为0.027至0.080[木村双参数距离（K2P）]，而种间变异值范围为0.049至0.491。发现长腰金枪鱼（T. tonggol）和黄鳍金枪鱼（T. albacares）关系密切（K2P = 0.049），而鲣（K. pelamis）是研究的物种中分歧最大的。使用最大似然法（ML）和邻接法（NJ）进行的系统发育分析支持金枪鱼属物种的单系起源。同样，扁舵鲣属和羽鳃鲔属物种的系统发育证实了单系性。然而，结果显示鲣与金枪鱼属以及扁舵鲣属和羽鳃鲔属有不同的起源。因此，mtDNA D环区域序列数据支持金枪鱼物种的多系起源。
