线粒体外膜中尿苷二磷酸葡萄糖：多萜醇单磷酸葡萄糖基转移酶的组织形式
Organization of mitochondrial UDP-glucose:dolichylmonophosphate glucosyltransferase in the outer membrane.
作者信息
Gasnier F, Lerme F, Vaganay E, Louisot P, Gateau-Roesch O
机构信息
University of Lyon, Lyon-Sud Medical School, Department of Biochemistry, INSERM U.189, CNRS.
出版信息
Biosci Rep. 1989 Dec;9(6):753-61. doi: 10.1007/BF01114814.
Previous studies have shown the existence of an autonomous mitochondrial UDP-glucose: dolichylmonophosphate glucosyltransferase, located in mitochondrial outer membrane of liver cells. To improve our knowledge about the topographical aspects of glycosylation in mitochondria, we have investigated the organization of this enzyme in intact mitochondria, using controlled proteolysis with trypsin and sensitivity towards amino-acid specific reagents. Our data provides evidence: --for a mitochondrial glucosyltransferase facing the cytoplasmic side of the outer membrane --and for the involvement of histidine and tryptophan residues as well as sulfhydryl groups in the catalytic activity of the enzyme.
先前的研究表明，在肝细胞线粒体外膜存在一种自主的线粒体UDP-葡萄糖：二磷酸多萜醇葡萄糖基转移酶。为了增进我们对线粒体中糖基化拓扑学方面的了解，我们利用胰蛋白酶进行可控蛋白水解以及对氨基酸特异性试剂的敏感性，研究了完整线粒体中这种酶的组织形式。我们的数据提供了证据：——证明存在一种面向外膜胞质侧的线粒体葡萄糖基转移酶——以及证明组氨酸、色氨酸残基和巯基参与了该酶的催化活性。
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