From the Molecular Bacteriology Laboratory, Pasteur Institute of Morocco , Casablanca.
Infect Dis (Lond). 2015 Jan;47(1):27-32. doi: 10.3109/00365548.2014.961542. Epub 2014 Oct 20.
The importance of community-acquired infections due to extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) has been increasingly recognized in recent years. This study aimed to determine the prevalence of intestinal carriage of ESBL-PE in the community in Casablanca, Morocco.
During 6 months (2013), 93 fecal samples were examined for ESBL-PE. Isolates expressing an ESBL phenotype were investigated for the presence of genes encoding β-lactamases and plasmid-mediated quinolone resistance. Conjugation experiments were done to determine the mobility of ESBL genes.
The prevalence of fecal carriage of ESBL-PE was 4.3% (4/93; 95% CI, 0.2-8.4). Klebsiella pneumoniae (n = 2), Enterobacter cloacae (n = 2), Escherichia coli (n = 1), and Serratia odorifera (n = 1) were the ESBL-producing species. Four (66.7%) of these isolates were multidrug-resistant. The blaSHV-12 (n = 5) was the most frequent ESBL gene detected, followed by blaCTX-M-15 (n = 3).The non-ESBL gene detected was blaTEM-1 (n = 5). One isolate harbored the qnrB1 variant. RESULTS of conjugation experiments indicated that blaSHV-12 + blaTEM-1 + qnrB1 and blaCTX-M-15 + blaTEM-1 genes were co-transferred and that these genes were carried by a conjugative plasmid of high molecular weight (125 kb).
Our results show the importance of the intestinal tract as a reservoir for ESBL-PE in the community in Morocco.
近年来，产超广谱β-内酰胺酶肠杆菌科（ESBL-PE）社区获得性感染的重要性日益受到关注。本研究旨在确定摩洛哥卡萨布兰卡社区中肠外产 ESBL-PE 的流行率。
在 6 个月（2013 年）期间，检查了 93 份粪便样本，以检测 ESBL-PE。对表现出 ESBL 表型的分离株进行了β-内酰胺酶和质粒介导的喹诺酮耐药基因的检测。进行了接合实验以确定 ESBL 基因的可移动性。
肠外产 ESBL-PE 的粪便携带率为 4.3%（4/93；95%CI，0.2-8.4）。产 ESBL 的物种包括肺炎克雷伯菌（n=2）、阴沟肠杆菌（n=2）、大肠埃希菌（n=1）和粘质沙雷菌（n=1）。其中 4 种（66.7%）为多药耐药菌。检测到的 blaSHV-12（n=5）是最常见的 ESBL 基因，其次是 blaCTX-M-15（n=3）。检测到的非 ESBL 基因是 blaTEM-1（n=5）。1 株菌携带 qnrB1 变体。接合实验的结果表明，blaSHV-12+blaTEM-1+qnrB1 和 blaCTX-M-15+blaTEM-1 基因被共转移，这些基因由高分子量（125kb）的接合质粒携带。
我们的结果表明，肠道是摩洛哥社区中 ESBL-PE 的重要储存库。
