Thorbert-Mros S, Larsson L, Berglundh T
Department of Periodontology, Institute of Odontology, The Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.
J Periodontal Res. 2015 Aug;50(4):535-43. doi: 10.1111/jre.12236. Epub 2014 Oct 20.
Insufficient information on the cellular composition of long-standing gingivitis lesions without signs of attachment loss makes an understanding of differences in cellular composition between "destructive" and "nondestructive" periodontal lesions difficult. The aim of the current study was to analyze differences in cell characteristics between lesions representing long-standing gingivitis and severe periodontitis.
Two groups of patients were recruited. One group consisted of 36 patients, 33-67 years of age, with severe generalized periodontitis (periodontitis group). The second group consisted of 28 patients, 41-70 years of age, with overt signs of gingival inflammation but no attachment loss (gingivitis group). From each patient a gingival biopsy was obtained from one selected diseased site and prepared for immunohistochemical analysis.
Periodontitis lesions were twice as large and contained significantly larger proportions, numbers and densities of cells positive for CD138 (plasma cells) and CD68 (macrophages) than did gingivitis lesions. The proportion of B cells that expressed the additional CD5 marker (B-1a cells) was significantly larger in periodontitis lesions than in gingivitis lesions. The densities of T cells and B cells did not differ between periodontitis lesions and gingivitis lesions. T cells were not the dominating cell type in gingivitis lesions, as B cells together with their subset plasma cells comprised a larger number and proportion than T cells.
Periodontitis lesions at teeth with advanced attachment and bone loss exhibit quantitative and qualitative differences in relation to gingivitis lesions at teeth with no attachment and bone loss. It is suggested that the large number and high density of plasma cells are the hallmarks of advanced periodontitis lesions and the most conspicuous difference in relation to long-standing gingivitis lesions.
关于无附着丧失迹象的长期牙龈炎病变细胞组成的信息不足，使得理解“破坏性”和“非破坏性”牙周病变之间的细胞组成差异变得困难。本研究的目的是分析代表长期牙龈炎和重度牙周炎的病变之间细胞特征的差异。
招募了两组患者。一组由36名年龄在33 - 67岁之间的重度广泛性牙周炎患者组成（牙周炎组）。第二组由28名年龄在41 - 70岁之间、有明显牙龈炎症迹象但无附着丧失的患者组成（牙龈炎组）。从每位患者的一个选定病变部位获取牙龈活检组织，并准备进行免疫组织化学分析。
牙周炎病变的大小是牙龈炎病变的两倍，并且CD138阳性（浆细胞）和CD68阳性（巨噬细胞）的细胞比例、数量和密度显著更高。表达额外CD5标志物的B细胞比例（B - 1a细胞）在牙周炎病变中比在牙龈炎病变中显著更大。牙周炎病变和牙龈炎病变之间T细胞和B细胞的密度没有差异。在牙龈炎病变中T细胞不是主要细胞类型，因为B细胞及其亚群浆细胞的数量和比例比T细胞更大。
有晚期附着丧失和骨丧失的牙齿的牙周炎病变与无附着丧失和骨丧失的牙齿的牙龈炎病变相比，在数量和质量上存在差异。提示浆细胞的大量存在和高密度是晚期牙周炎病变的标志，也是与长期牙龈炎病变最显著的差异。
