Hu Wensheng, Wang Hanzhi, Huang Hefeng
Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
J Obstet Gynaecol Res. 2015 Mar;41(3):343-9. doi: 10.1111/jog.12555. Epub 2014 Oct 20.
The aim of this study was to determine the promoter methylation status of type 2 isoform of 11β-hydroxysteroid dehydrogenase (11β-HSD2) and its regulatory correlation with 11β-HSD2 gene expression in placentas of pre-eclampsia (PE) patients of Chinese Han ethnicity.
The pathological features of placental tissues were studied using hematoxylin-eosin staining and immunohistochemical staining. The 11β-HSD2 mRNA and protein expressions were detected by real-time polymerase chain reaction and Western blotting. The methylation of the 11β-HSD2 promoter sequence was examined by bisulfite sequencing polymerase chain reaction.
Trophoblast hyperplasia and discontinuous syncytial layer were observed in the PE group, and the 11β-HSD2 was distributed irregularly and its immunoreactivity was weakened distinctly. The expressions of 11β-HSD2 mRNA and protein decreased significantly in the PE group compared with the control group. Unexpectedly, almost no 11β-HSD2 methylation was detected in PE placental tissue (two fragments, 0.6% vs 0%) or normal placental tissue (1% vs 0.6%). No significant difference in 11β-HSD2 promoter methylation was found between the two groups.
The 11β-HSD2 expression decreased in PE women of Chinese Han ethnicity, but was not interrelated with the promoter methylation status.
本研究旨在确定中国汉族子痫前期（PE）患者胎盘组织中11β-羟基类固醇脱氢酶2型（11β-HSD2）启动子甲基化状态及其与11β-HSD2基因表达的调控关系。
采用苏木精-伊红染色和免疫组织化学染色研究胎盘组织的病理特征。通过实时聚合酶链反应和蛋白质免疫印迹法检测11β-HSD2 mRNA和蛋白表达。采用亚硫酸氢盐测序聚合酶链反应检测11β-HSD2启动子序列的甲基化情况。
PE组可见滋养层细胞增生和合体滋养层不连续，11β-HSD2分布不规则，免疫反应性明显减弱。与对照组相比，PE组11β-HSD2 mRNA和蛋白表达显著降低。出乎意料的是，在PE胎盘组织（两个片段，0.6%对0%）或正常胎盘组织（1%对0.6%）中几乎未检测到11β-HSD2甲基化。两组间11β-HSD2启动子甲基化无显著差异。
中国汉族PE女性中11β-HSD2表达降低，但与启动子甲基化状态无关。
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