Optics and Imaging Centre, School of Laboratory Medicine and Medical Sciences, Nelson Mandela Medical School, University of KwaZulu-Natal, Durban, South Africa; Department of Anatomy, Obafemi Awolowo College of Health Sciences, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria.
Optics and Imaging Centre, School of Laboratory Medicine and Medical Sciences, Nelson Mandela Medical School, University of KwaZulu-Natal, Durban, South Africa.
J Reprod Immunol. 2020 Nov;142:103185. doi: 10.1016/j.jri.2020.103185. Epub 2020 Aug 13.
Preeclampsia (PE) and human immunodeficiency virus (HIV) have been linked with marked increases in maternal stress, resulting in a significant change in placental function ranging from alterations in placental structure to the precise and delicate transformations in placental gene expression. Such changes may lead to altered transport of essential signals to the fetus, which can have long-term impacts on offspring health and consequently affect fetal neurodevelopment. Therefore, this work investigated the role of placental 11β-hydroxysteroid dehydrogenase types 2 (11β-HSD2) in HIV associated preeclampsia. The placenta were obtained from 76 pregnant women, which were stratified based on pregnancy type and HIV status into; Normotensive HIV negative, normotensive HIV positive, PE HIV negative and PE HIV positive. The placental tissue was processed for immunocytochemistry and stained with rabbit polyclonal to 11β-HSD2 Our results showed significant downregulation in the placental expression of 11β-HSD2 in both the conducting and exchange villi of PE and HIV-positive patients when compared with Normotensive and HIV-negative individuals, respectively. Our results provide inferential evidence for comorbidity of PE and HIV in the downregulation of placental 11β-HSD2 enzyme function, which mediates the programmed outcomes of an adverse maternal environment during pregnancy and long-term impacts on offspring health and consequently affects fetal neurodevelopment.
子痫前期 (PE) 和人类免疫缺陷病毒 (HIV) 与母体应激的显著增加有关，导致胎盘功能发生显著变化，从胎盘结构的改变到胎盘基因表达的精确和微妙变化。这些变化可能导致必需信号向胎儿的转运发生改变，从而对后代的健康产生长期影响，并因此影响胎儿的神经发育。因此，本研究探讨了胎盘 11β-羟类固醇脱氢酶 2 型 (11β-HSD2) 在与 HIV 相关的子痫前期中的作用。从 76 名孕妇中获取胎盘，根据妊娠类型和 HIV 状态将其分层为：正常血压 HIV 阴性、正常血压 HIV 阳性、PE HIV 阴性和 PE HIV 阳性。胎盘组织进行免疫细胞化学处理，并使用兔多克隆抗体对 11β-HSD2 进行染色。我们的结果表明，与正常血压和 HIV 阴性个体相比，PE 和 HIV 阳性患者的胎盘绒毛和交换绒毛中 11β-HSD2 的表达均显著下调。我们的结果提供了推论性证据，证明 PE 和 HIV 的合并症与胎盘 11β-HSD2 酶功能的下调有关，该酶介导了妊娠期间不利母体环境的程序化结局，并对后代的健康产生长期影响，从而影响胎儿的神经发育。
