不对称巨型脂质囊泡制备

Asymmetric giant lipid vesicle fabrication.

作者信息

Hu Peichi C, Malmstadt Noah

机构信息

Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, 925 Bloom Walk, HED 216, Los Angeles, CA, 90089-1211, USA.

出版信息

Methods Mol Biol. 2015;1232:79-90. doi: 10.1007/978-1-4939-1752-5_7.

DOI:10.1007/978-1-4939-1752-5_7

PMID:25331129

Abstract

Synthetic lipid bilayers have long been used as models of cell membranes. The compositional asymmetry in the eukaryotic plasma membrane is a key chemical characteristic of this membrane that has traditionally been difficult to reproduce in synthetic systems. In this chapter, we describe recent technologies for fabricating compositionally asymmetric giant unilamellar lipid vesicles (GUVs) and provide detailed protocols for a microfluidic-based fabrication technique.

摘要

合成脂质双层长期以来一直被用作细胞膜的模型。真核细胞质膜中的组成不对称性是该膜的一个关键化学特征，传统上在合成系统中很难再现。在本章中，我们描述了用于制造组成不对称的巨型单层脂质囊泡（GUVs）的最新技术，并提供了基于微流体的制造技术的详细方案。