Photonics and Optoelectronics Group, Physics Department and CeNS, Ludwig-Maximilians-Universität München, Amalienstr. 54, 80799 Munich, Germany.
J Biophotonics. 2012 Jan;5(1):40-6. doi: 10.1002/jbio.201100058.
Microfluidic jetting is a promising method to produce giant unilamellar phospholipid vesicles for mimicking living cells in biomedical studies. We have investigated the chemical composition of membranes of vesicles prepared using this approach by means of Raman scattering spectroscopy. The membranes of all jetted vesicles are found to contain residuals of the organic solvent decane used in the preparation of the initial planar membrane. The decane inclusions are randomly distributed over the vesicle surface area and vary in thickness from a few to several tens of nanometers. Our findings point out that the membrane properties of jetted vesicles may differ considerably from those of vesicles prepared by other methods and from those of living cells.
微流喷射是一种有前途的方法，可以生产用于模拟生物医学研究中活细胞的巨大单层磷脂囊泡。我们通过拉曼散射光谱研究了使用这种方法制备的囊泡膜的化学成分。发现所有喷射囊泡的膜都含有在制备初始平面膜时使用的有机溶剂癸烷的残留物。癸烷包含物随机分布在囊泡表面积上，厚度从几纳米到几十纳米不等。我们的发现指出，喷射囊泡的膜性质可能与其他方法制备的囊泡以及活细胞的膜性质有很大的不同。
