Yu Jia-Ling, Ma Li, Ma Lan, Tao Ye-Zheng
The State Key Laboratory of Medical Neurobiology and Pharmacology Research Center, Shanghai Medical College and Institutes of Brain Science, Fudan University, Shanghai 200032, China.
Sheng Li Xue Bao. 2014 Oct 25;66(5):559-68.
Adult hippocampal neurogenesis plays important roles in learning, memory and mood regulation. External factors, such as physical exercise, have been found to modulate adult hippocampal neurogenesis. Voluntary running enhances cell proliferation in subgranular zone (SGZ) and increases the number of new born neurons in rodents, but underlying mechanisms are not fully understood. In this study, we used BrdU assay to identify proliferating cells in 2-month-old C57BL/6 mice after 15 days of voluntary wheel running test. mRNA and protein levels for several neural factors in dentate gyrus, Ammon's horn, and cortex were also analyzed by RT-qPCR and Western blot assay after 15 days of voluntary wheel running. Our data show that voluntary wheel running for 15 days elevated the number of proliferation cells in dentate gyrus and significantly up-regulated the mRNA levels of Bdnf, Igf1 and Wnt4. The protein levels of BDNF and IGF1 in dentate gyrus were also increased after voluntary wheel running. These results indicate that the increase of adult hippocampal neurogenesis caused by voluntary wheel running for 15 days might be through up-regulating BDNF, IGF1 and WNT4 in dentate gyrus.
成年海马神经发生在学习、记忆和情绪调节中发挥着重要作用。已发现诸如体育锻炼等外部因素可调节成年海马神经发生。自愿跑步可增强颗粒下区（SGZ）的细胞增殖，并增加啮齿动物新生神经元的数量，但其潜在机制尚未完全明确。在本研究中，我们使用BrdU检测法在2个月大的C57BL/6小鼠进行15天的自愿转轮测试后鉴定增殖细胞。在自愿转轮15天后，还通过RT-qPCR和蛋白质印迹分析检测齿状回、海马角和皮质中几种神经因子的mRNA和蛋白质水平。我们的数据表明，15天的自愿转轮增加了齿状回中增殖细胞的数量，并显著上调了Bdnf、Igf1和Wnt4的mRNA水平。自愿转轮后齿状回中BDNF和IGF1的蛋白质水平也有所增加。这些结果表明，15天的自愿转轮所导致的成年海马神经发生增加可能是通过上调齿状回中的BDNF、IGF1和WNT4来实现的。
