González-Gutiérrez Alejandra G, Gutiérrez-Mora Antonia, Rodríguez-Garay Benjamín
Unidad de Biotecnología Vegetal, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. (CIATEJ), Av. Normalistas No. 800, Col. Colinas de la Normal, Guadalajara, Jalisco 44270 México.
Springerplus. 2014 Oct 1;3:575. doi: 10.1186/2193-1801-3-575. eCollection 2014.
Agave tequilana is an angiosperm species that belongs to the family Asparagaceae (formerly Agavaceae). Even though there is information regarding to some aspects related to the megagametogenesis of A. tequilana, this is the first report describing the complete process of megasporogenesis, megagametogenesis, the early embryo and endosperm development process in detail. The objective of this work was to study and characterize all the above processes and the distinctive morphological changes of the micropylar and chalazal extremes after fertilization in this species. The agave plant material for the present study was collected from commercial plantations in the state of Jalisco, Mexico. Ovules and immature seeds, previously fixed in FAA and kept in ethanol 70%, were stained based on a tissue clarification technique by using a Mayer's-Hematoxylin solution. The tissue clarification technique was successfully used for the characterization of the megasporogenesis, megagametogenesis, mature embryo sac formation, the early embryo and endosperm development processes by studying intact cells. The embryo sac of A. tequilana was confirmed to be of the monosporic Polygonum-type and an helobial endosperm formation. Also, the time-lapse of the developmental processes studied was recorded.
龙舌兰是一种被子植物，属于天门冬科（原龙舌兰科）。尽管有关于龙舌兰大孢子发生某些方面的信息，但这是第一份详细描述大孢子发生、大配子发生、早期胚胎和胚乳发育过程的完整过程的报告。这项工作的目的是研究和表征上述所有过程以及该物种受精后珠孔端和合点端的独特形态变化。本研究的龙舌兰植物材料采自墨西哥哈利斯科州的商业种植园。先前固定在FAA中并保存在70%乙醇中的胚珠和未成熟种子，使用梅耶苏木精溶液基于组织透明技术进行染色。通过研究完整细胞，组织透明技术成功用于表征大孢子发生、大配子发生、成熟胚囊形成、早期胚胎和胚乳发育过程。龙舌兰的胚囊被确认为单孢子蓼型和沼生目型胚乳形成。此外，还记录了所研究发育过程的时间推移。
