Yoda Y, Mori N, Oka K, Nakazawa M, Mukai R, Abe T
Nihon Ketsueki Gakkai Zasshi. 1989 Jul;52(4):740-8.
The intracytoplasmic localization of CD3 antigen was examined in azurophilic granular T-lymphoblastic lymphoma cells obtained from a 27-year-old female. Examination by flow cytometry and an immunohistological method revealed that the surface phenotype of the lymphoma cells were NKH1+, CD5+, CD7+, CD1-, CD2-, CD4-, CD8- and CD16-. CD3 was not detected on the cell-surface by flow cytometry but was found in the cytoplasm using the immunohistological method. CD3 was detected in the perinuclear space and rough endoplasmic reticulum (rough ER) by an immunoelectron microscopic study. An electron microscopic observation of the granules revealed lysosomal structures with low electron density. The lymphoma cells proliferated when cultured in the presence of interleukin-2 but showed no NK (K562) activity before and after culture. The molecular size of metabolically labeled intracytoplasmic CD3 precipitated by Leu 4 monoclonal antibody was identical to that of normal CD3. These results indicate that the lymphoma cells are neoplastic counterparts of immature thymocyte at a very early stage of differentiation and may be related to the lineage of CD3+ NKH1+ T-lymphocyte.
对一名27岁女性的嗜天青颗粒性T淋巴细胞母细胞瘤细胞中的CD3抗原进行了胞浆内定位研究。通过流式细胞术和免疫组织学方法检查发现，淋巴瘤细胞的表面表型为NKH1 +、CD5 +、CD7 +、CD1 -、CD2 -、CD4 -、CD8 -和CD16 -。流式细胞术未在细胞表面检测到CD3，但免疫组织学方法发现其存在于细胞质中。免疫电子显微镜研究显示，CD3存在于核周间隙和粗面内质网（糙面内质网）中。对颗粒的电子显微镜观察显示出低电子密度的溶酶体结构。淋巴瘤细胞在白细胞介素-2存在的情况下培养时会增殖，但培养前后均未表现出NK（K562）活性。由Leu 4单克隆抗体沉淀的代谢标记胞浆内CD3的分子大小与正常CD3相同。这些结果表明，淋巴瘤细胞是分化非常早期的未成熟胸腺细胞的肿瘤对应物，可能与CD3 + NKH1 + T淋巴细胞谱系有关。
