Goll F
National Veterinary Services Laboratories, U.S. Department of Agriculture, Ames, Iowa 50010.
Avian Dis. 1989 Oct-Dec;33(4):760-3.
A national survey was conducted to address the concern over the uniformity of serological test results for avian mycoplasmosis. The National Veterinary Services Laboratories produced chicken and turkey mycoplasma serum-check test kits. Each kit had a total of 25 sera that consisted of negative and positive sera. Participating laboratories were requested to examine their kits using the serum plate agglutination test with the plate antigens currently being used and the hemagglutination inhibition test with the hemagglutinating antigens provided. A conclusion whether serum plate agglutination-positive sera were positive, negative, or suspicious was based on the hemagglutination inhibition test results. Results in each category were scored on the basis of 100 points. The average scores on the serum plate agglutination test, hemagglutination inhibition test, and conclusion were 95, 71, and 91, respectively, for the chicken serum test kit and 91, 71, and 89, respectively, for the turkey serum test kit. The results indicated a high degree of uniformity among laboratories in reporting serological test results for mycoplasmosis in chickens and turkeys.
为了解决对禽支原体病血清学检测结果一致性的担忧，开展了一项全国性调查。国家兽医服务实验室生产了鸡和火鸡支原体血清检测试剂盒。每个试剂盒共有25份血清，包括阴性和阳性血清。要求参与实验室使用当前正在使用的平板抗原通过血清平板凝集试验以及使用提供的血凝抗原通过血凝抑制试验来检测其试剂盒。血清平板凝集试验阳性血清是阳性、阴性还是可疑的结论基于血凝抑制试验结果。每个类别中的结果以100分为基础进行评分。鸡血清检测试剂盒的血清平板凝集试验、血凝抑制试验和结论的平均得分分别为95分、71分和91分，火鸡血清检测试剂盒的平均得分分别为91分、71分和89分。结果表明，各实验室在报告鸡和火鸡支原体病血清学检测结果方面具有高度的一致性。
