Frolova Ya N, Kharseyeva G G, Mironov A Yu
Klin Lab Diagn. 2014 Jun(6):51-3.
The article presents analysis of sensitivity to antibacterial preparations of typical and biofilm culture of museum strain of Corynebacterium diphtheriae gravis tox+ SV-665. The strain was obtained from the L.A. Tarasevitch state research institute of standardization and control of medical biological preparations. The second strain C. diphtheriaecirculates gravis tox+ circulates in population of the Rostov oblast and it was recovered from patient with diagnosis of "localized form of diphtheria" by bacteriologic laboratory "1002 CGSEN SKVO" of Rostov-on-Don. The week and month biofilm cultures of both strains of C. diphtheriae gravis tox+ were used. The sensitivity to antibacterial preparations of typical and biofilm cultures of museum and circulating in population strains of agent of diphtheria were detected using minimal suppressing concentration by technique of serial dilutions in fluid growth medium. It is demonstrated that the most effective in respect of C. diphtheriae are such preparations as cefotaxinum, gentamycinum, lincomycin, canamycin and cefasolin. The sensitivity of pathogen in composition of biofilm to these preparations has no changes.
本文介绍了对重症白喉棒状杆菌博物馆菌株典型培养物和生物膜培养物对抗菌制剂敏感性的分析，该菌株为tox + SV - 665型。该菌株取自俄罗斯国家医学和生物制剂标准化与控制研究所L.A.塔拉谢维奇。第二株重型白喉棒状杆菌tox +在罗斯托夫州人群中传播，它是由顿河畔罗斯托夫的“1002 CGSEN SKVO”细菌学实验室从诊断为“局限性白喉”的患者身上分离得到的。使用了两株重型白喉棒状杆菌tox +的一周和一个月生物膜培养物。通过在液体生长培养基中连续稀释技术，采用最低抑菌浓度检测白喉病原体博物馆菌株和人群中传播菌株的典型培养物及生物膜培养物对抗菌制剂的敏感性。结果表明，对于白喉棒状杆菌最有效的制剂是头孢噻肟、庆大霉素、林可霉素、卡那霉素和头孢唑林。病原体在生物膜中的组成对这些制剂的敏感性没有变化。
