Bailey L B, Molloy A, Scott J, Rice D
Food Science and Human Nutrition Department, University of Florida, Gainesville 32611.
J Inherit Metab Dis. 1989;12(4):429-35. doi: 10.1007/BF01802038.
The streptozotocin-treated diabetic rat was not found to be a suitable animal model for methylmalonic acidaemia as previously described. Urinary methylmalonic acid (MMA) was measured in adult Wistar rats prior to and following injection of streptozotocin (40 mg/kg). Plasma and tissue MMA levels were measured following the induction of diabetes and compared with data from control rats. MMA levels were determined by a gas chromatographic-mass spectrometric method (McMurray et al., 1986). Diabetes was confirmed by the 10 x increase in 24 h urine volume; glycosuria; glycaemia; and weight loss. Urinary MMA excreted did not change during the 11 week diabetic period and there was no difference between the pre- and post-diabetic phases (p less than 0.05). Plasma and tissue MMA concentrations were not elevated in this diabetic animal model. Also in contrast to earlier reports, the vitamin B12 levels of the diabetic rats were not elevated compared to controls (p less than 0.05).
如先前所述，链脲佐菌素诱导的糖尿病大鼠并非甲基丙二酸血症的合适动物模型。在成年Wistar大鼠注射链脲佐菌素（40 mg/kg）之前和之后测量尿甲基丙二酸（MMA）。在诱导糖尿病后测量血浆和组织MMA水平，并与对照大鼠的数据进行比较。MMA水平通过气相色谱 - 质谱法测定（McMurray等人，1986年）。通过24小时尿量增加10倍、糖尿、血糖和体重减轻来确认糖尿病。在11周的糖尿病期间，尿中排出的MMA没有变化，糖尿病前期和后期之间没有差异（p小于0.05）。在这个糖尿病动物模型中，血浆和组织MMA浓度没有升高。同样与早期报告相反，糖尿病大鼠的维生素B12水平与对照组相比没有升高（p小于0.05）。
