Bhatt H R, Green A, Linnell J C
Clin Chim Acta. 1982 Feb 5;118(2-3):311-21. doi: 10.1016/0009-8981(82)90018-3.
A routine method for the rapid estimation of methylmalonic acid (MMA) has been developed using thin-layer chromatography (TLC) on cellulose, locating the separated MMA zones by coupling with a diazo reagent and quantitating the results by densitometric scanning of a photocopy of the chromatogram on transparency film. The method can detect as little as 25 pg MMA in small volumes of urine, plasma and other body fluids or tissue homogenates, has potential for the simultaneous estimation of MMA, other organic acids, methylmalonyl coenzyme A and creatinine and should be of particular value in the investigation of occult cobalamin deficiency or suspected errors of vitamin B12 or intermediary metabolism.
已开发出一种快速估算甲基丙二酸（MMA）的常规方法，该方法利用纤维素薄层层析（TLC），通过与重氮试剂偶联来定位分离出的MMA区带，并通过对透明胶片上色谱图复印件进行光密度扫描来定量结果。该方法可检测少量尿液、血浆及其他体液或组织匀浆中低至25皮克的MMA，有潜力同时估算MMA、其他有机酸、甲基丙二酰辅酶A和肌酐，在隐匿性钴胺素缺乏或疑似维生素B12或中间代谢错误的调查中应具有特殊价值。
