Liu Haibo, Yang Shunzhang, Sun Xuejun, Chen Tianbao
Department of Cardiovascular Surgery, Quanzhou First Hospital of Fujian Medical University Quanzhou 362000, China.
Department of Cardiology, Quanzhou First Hospital of Fujian Medical University Quanzhou 362000, China.
Int J Clin Exp Pathol. 2014 Aug 15;7(9):5864-71. eCollection 2014.
The aim this study is to explore effect of IL-10 on apoptosis of VSMCs in allograft arterial transplantation rats, and to investigate mechanism. SD rats were divied into three groups, including control group (CN, with physiological saline), blank vector group (BV, with blank adenovirus) and combined gene group (CG, with adenovirus carried IL-10 gene). The isolated donor vascular was transfected with the adenovirus carried hIL-10 gene for 30 minutes by immersing method. Forty-five days post transplantation, the grafts were harvested. The allografts pathologioc changes were observed and the size of vascular intima and middle layer of allografts were measured. The expression of hIL-10 was detected by RT-PCR, ELISA and immunohistochemistry, respectively. The repression of Fas/Fasl in artery allografts was also examined by immunohistochemistry method. The results indicated that 45 days after transplantation, the intimal and middle hyperplasia ratio in CG group was significantly lower than that in CN and BV group (P < 0.05). The transgene expression of human interleukin-10 was significantly enhanced in CG group compared to CN and BV group by ELISA, RT-PCR and immunohistochemistry (P < 0.05). The expression of Fas/FasL was higher in CG group compared with the other groups (P < 0.05). The level of apoptotic smooth muscle cells were significantly increased in CG group compared to CN and BV group (P < 0.05). In conclusion, adenovirus mediated IL-10 expression could up-regulate Fas/FasL expression, induce smooth muscle cell apoptosis and alleviate angiosclerosis process. The IL-10 gene transfer to allograft artery could inhibit acute rejection reaction of allograft vascular transplantation.
本研究旨在探讨白细胞介素-10（IL-10）对同种异体动脉移植大鼠血管平滑肌细胞（VSMCs）凋亡的影响，并研究其机制。将SD大鼠分为三组，包括对照组（CN，注射生理盐水）、空白载体组（BV，注射空白腺病毒）和联合基因组（CG，注射携带IL-10基因的腺病毒）。采用浸泡法将携带人IL-10基因的腺病毒转染至分离的供体血管30分钟。移植后45天，获取移植物。观察移植物的病理变化，并测量移植物血管内膜和中层的大小。分别采用逆转录-聚合酶链反应（RT-PCR）、酶联免疫吸附测定（ELISA）和免疫组织化学法检测hIL-10的表达。同时采用免疫组织化学法检测动脉移植物中Fas/Fas配体（FasL）的表达情况。结果显示，移植后45天，CG组内膜和中层增生率显著低于CN组和BV组（P<0.05）。ELISA、RT-PCR和免疫组织化学检测结果显示，与CN组和BV组相比，CG组人白细胞介素-10的转基因表达显著增强（P<0.05）。与其他组相比，CG组Fas/FasL的表达更高（P<0.05）。与CN组和BV组相比，CG组凋亡平滑肌细胞水平显著升高（P<0.05）。综上所述，腺病毒介导的IL-10表达可上调Fas/FasL表达，诱导平滑肌细胞凋亡，减轻血管硬化进程。将IL-10基因转导至同种异体动脉可抑制同种异体血管移植的急性排斥反应。
