Zarbakhsh Sam, Moradi Fatemeh, Joghataei Mohammad Taghi, Bahktiari Mehrdad, Mansouri Korosh, Abedinzadeh Mahmood
Department of Anatomy, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Department of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran ; Cellular and Molecular Research Center, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Basic Clin Neurosci. 2013 Fall;4(4):291-8.
Transplantation of bone marrow stromal cells (BMSCs) or Schwann cells (SCs) can increase axonal regeneration in peripheral nerve injuries. Based on our previous investigations, the goal of the present work was to examine the individual and synergistic effects of the two different cell types in sciatic nerve injury. We pursued to evaluate the effects of BMSCs and SCs co-transplantation on the functional recovery after sciatic nerve injury in rat.
In this experimental research, adult male Wistar rats (n = 32, 250-300g) were used, BMSCs and SCs were cultured, and the SCs were confirmed with anti S100 antibody. Rats were randomly divided into 4 groups (n = 8 in each group): 1-control group: silicon tube filled with fibrin gel without cells; 2-BMSCs group: silicon tube filled with fibrin gel seeded with BMSCs; 3-SCs group: silicon tube filled with fibrin gel seeded with SCs and 4-co-transplantation group: silicone tube filled with fibrin gel seeded with BMSCs and SCs. The left sciatic nerve was exposed, a 10 mm segment removed, and a silicone tube interposed into this nerve gap. BMSCs and SCs were transplanted separately or in combination into the gap. BMSCs were labeled with anti-BrdU and SCs were labeled with DiI. After 12 weeks electromyographic and functional assessments were performed and analyzed by one-way analysis of variance (ANOVA).
Electromyographic and functional assessments showed a significant difference between the experimental groups and controls. Electromyography measures were significantly more favourable in SCs transplantation group as compared to BMSCs transplantation and co-transplantation groups (p < 0.05). Functional assessments showed no statistically significant difference among the BMSCs, SCs and co-transplantation groups (p < 0.05).
Transplantation of BMSCs and SCs separately or in combination have the potential to generate functional recovery after sciatic nerve injury in rat. The electromyography evaluation showed a greater improvement after SCs transplantation than BMSCs or the co-transplantation of BMSCs and SCs.
骨髓基质细胞（BMSCs）或雪旺细胞（SCs）移植可促进周围神经损伤后的轴突再生。基于我们之前的研究，本研究的目的是检测这两种不同细胞类型在坐骨神经损伤中的单独及协同作用。我们旨在评估BMSCs和SCs联合移植对大鼠坐骨神经损伤后功能恢复的影响。
在本实验研究中，使用成年雄性Wistar大鼠（n = 32，体重250 - 300g），培养BMSCs和SCs，并用抗S100抗体鉴定SCs。大鼠随机分为4组（每组n = 8）：1 - 对照组：填充有不含细胞的纤维蛋白凝胶的硅胶管；2 - BMSCs组：填充有接种BMSCs的纤维蛋白凝胶的硅胶管；3 - SCs组：填充有接种SCs的纤维蛋白凝胶的硅胶管；4 - 联合移植组：填充有接种BMSCs和SCs的纤维蛋白凝胶的硅胶管。暴露左侧坐骨神经，切除10mm节段，在神经缺损处插入硅胶管。将BMSCs和SCs单独或联合移植到缺损处。用抗BrdU标记BMSCs，用DiI标记SCs。12周后进行肌电图和功能评估，并通过单因素方差分析（ANOVA）进行分析。
肌电图和功能评估显示实验组与对照组之间存在显著差异。与BMSCs移植组和联合移植组相比，SCs移植组的肌电图测量结果明显更优（p < 0.05）。功能评估显示BMSCs组、SCs组和联合移植组之间无统计学显著差异（p < 0.05）。
单独或联合移植BMSCs和SCs有可能使大鼠坐骨神经损伤后实现功能恢复。肌电图评估显示，SCs移植后的改善比BMSCs移植或BMSCs与SCs联合移植更大。
