Zurita Mercedes, Bonilla Celia, Otero Laura, Aguayo Concepción, Vaquero Jesús
Neuroscience Research Unit, Puerta de Hierro Hospital, Department of Surgery, Autonomous University, San Martin de Porres, 4, 28035 Madrid, Spain.
Neurosci Res. 2008 Mar;60(3):275-80. doi: 10.1016/j.neures.2007.11.006. Epub 2007 Nov 22.
Bone marrow stromal cells (BMSC) can acquire morphological and immunohistochemical features of neural cells when they are treated with diverse chemical agents, a finding interpreted as result of cell transdifferentiation. With the purpose of a better knowledge of the possible utility of BMSC for strategies of Nervous System (NS) repair, we have studied the morphological and immunohistochemical changes induced in BMSC by chemical agents, in comparison with those that happen when BMSC are co-cultured with Schwann cells. While chemical BMSC transdifferentiation is a short-time reversible phenomenon, BMSC transdifferentiation obtained by Schwann cell-derived neurotrophic factors remains stable after it has been reached. These findings question the possible clinical utility of BMSC trandifferentiation using chemical agents, and support that neural transdifferentiation of BMSC is a biological phenomenon that can be obtained in vivo because of the presence of environmental factors.
骨髓基质细胞（BMSC）在用多种化学试剂处理时可获得神经细胞的形态学和免疫组化特征，这一发现被解释为细胞转分化的结果。为了更好地了解BMSC在神经系统（NS）修复策略中的潜在用途，我们研究了化学试剂诱导BMSC发生的形态学和免疫组化变化，并与BMSC与雪旺细胞共培养时发生的变化进行了比较。虽然化学诱导的BMSC转分化是一种短期可逆现象，但由雪旺细胞衍生的神经营养因子诱导的BMSC转分化在达到后仍保持稳定。这些发现对使用化学试剂进行BMSC转分化的潜在临床应用提出了质疑，并支持BMSC的神经转分化是一种由于环境因素的存在而可在体内发生的生物学现象。
