利用点击修饰的Pt(II)配合物方便地检测金属-DNA、金属-RNA和金属-蛋白质加合物。
Convenient detection of metal-DNA, metal-RNA, and metal-protein adducts with a click-modified Pt(II) complex.
作者信息
Moghaddam Alan D, White Jonathan D, Cunningham Rachael M, Loes Andrea N, Haley Michael M, DeRose Victoria J
机构信息
Department of Chemistry & Biochemistry, University of Oregon, Eugene, OR 97403-1253, USA.
出版信息
Dalton Trans. 2015 Feb 28;44(8):3536-9. doi: 10.1039/c4dt02649g.
cis-[Pt(2-azido-1,3-propanediamine)Cl2] is a reagent for high-yield post-treatment fluorescent labelling of Pt(II) biomolecular targets using click chemistry and exhibits a bias in conformational isomers in the context of duplex DNA. Pt-protein adducts are detected using BSA as a model. Following in vivo treatment, long-lived Pt-RNA adducts are detected on ribosomal RNA.
顺式-[Pt(2-叠氮基-1,3-丙二胺)Cl₂]是一种用于利用点击化学对Pt(II)生物分子靶标进行高产率后处理荧光标记的试剂，并且在双链DNA环境中构象异构体存在偏向性。以牛血清白蛋白为模型检测Pt-蛋白质加合物。体内处理后，在核糖体RNA上检测到长寿命的Pt-RNA加合物。
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