Han Xiao, Zhou Dao-Bin, Duan Ming-Hui, Hua Bao-Lai, Wang Xuan, Zhang Jie-Ping, Zhao Yong-Qiang, Shen Ti, Wu Yong-Ji
Department of Hematology, Peking Union Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
Department of Hematology, Peking Union Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2014 Oct;22(5):1316-20. doi: 10.7534/j.issn.1009-2137.2014.05.025.
This study was aimed to explore the possible mechanisms of hepcidin increase in multiple myeloma patients. The clinical information and peripheral venous blood of eligible patients with previously untreated multiple myeloma were collected. Serum concentration of IL-6 was detected by ELISA. Peripheral blood monocytes were isolated by CD14⁺ magnetic beads. The expression of hepcidin, IL-6 and C/EBPα mRNA of monocytes were detected by real time quantitative PCR. The results indicated that the hemoglobin level was reduced in 17 multiple myeloma patients enrolled in study (97.8 ± 27.5 g/L), showing the characteristics of anemia of chronic disease. The hepcidin and C/EBPα expression of peripheral blood monocytes significantly increased (P < 0.01), serum IL-6 was also higher than that in normal controls (P < 0.01). Serum IL-6 positively correlated with monocyte hepcidin and C/EBPα expression (P < 0.05); monocyte C/EBPα expression positively correlated with monocyte hepcidin expression (P < 0.05). It is concluded that the elevated IL-6 may induce hepcidin expression through up-regulating C/EBPα in untreated myeloma patients.
本研究旨在探讨多发性骨髓瘤患者铁调素升高的可能机制。收集符合条件的初治多发性骨髓瘤患者的临床资料及外周静脉血。采用酶联免疫吸附测定法检测血清白细胞介素-6（IL-6）浓度。通过CD14⁺磁珠分离外周血单核细胞。采用实时定量聚合酶链反应检测单核细胞中铁调素、IL-6及C/EBPα信使核糖核酸的表达。结果显示，纳入研究的17例多发性骨髓瘤患者血红蛋白水平降低（97.8±27.5g/L），呈现慢性病贫血特征。外周血单核细胞铁调素及C/EBPα表达显著升高（P<0.01），血清IL-6也高于正常对照组（P<0.01）。血清IL-6与单核细胞铁调素及C/EBPα表达呈正相关（P<0.05）；单核细胞C/EBPα表达与单核细胞铁调素表达呈正相关（P<0.05）。结论是，在未治疗的骨髓瘤患者中，升高的IL-6可能通过上调C/EBPα诱导铁调素表达。
