Damon L E, Christensen S, Rochlitz C, Cadman E C
Cancer Research Institute, University of California, San Francisco 94143.
Anticancer Res. 1989 Nov-Dec;9(6):1761-7.
The cytotoxic interactions between etoposide and two fluoropyrimidines (FP), 5-fluorouracil (FUra) and 5-fluoro-2'-deoxyuridine (FdUrd), were determined in L1210 cells by soft agar clonogenic assay and in five human adenocarcinoma cell lines by colony growth assay. The administration of etoposide prior to FP resulted in synergistic cytotoxicity in L1210, HCT-8, Mia PaCa, MCF-7, and T47-D cells while the reverse sequence resulted in additive or antagonistic cytotoxicity in L1210, HCT-8, and Mia PaCa cells. Etoposide prior to FdUrd produced more DNA single strand breaks than expected in L1210 cells, while the reverse sequence produced fewer than expected. The sequence--dependent synergistic cytotoxicity of etoposide--FP correlates with relative DNA single strand break production in L1210 cells.
通过软琼脂克隆形成试验在L1210细胞中以及通过集落生长试验在五种人腺癌细胞系中测定了依托泊苷与两种氟嘧啶（FP），即5-氟尿嘧啶（FUra）和5-氟-2'-脱氧尿苷（FdUrd）之间的细胞毒性相互作用。在FP之前给予依托泊苷在L1210、HCT-8、Mia PaCa、MCF-7和T47-D细胞中产生协同细胞毒性，而相反的给药顺序在L1210、HCT-8和Mia PaCa细胞中产生相加或拮抗细胞毒性。在FdUrd之前给予依托泊苷在L1210细胞中产生的DNA单链断裂比预期的多，而相反的顺序产生的则比预期的少。依托泊苷-FP的顺序依赖性协同细胞毒性与L1210细胞中相对DNA单链断裂的产生相关。
