游离钙离子对阿尔茨海默病和成纤维细胞中[钙离子 + 镁离子]依赖的三磷酸腺苷酶（ATP酶）的影响。

Effects of free Ca2+ on the [Ca2+ + Mg2+]-dependent adenosinetriphosphatase (ATPase) of Alzheimer and normal fibroblasts.

作者信息

Rizopoulos E, Chambers J P, Wayner M J, Martinez A O, Armstrong L S

机构信息

Brain Research Laboratory of Biochemistry, University of Texas, San Antonio 78285.

出版信息

Neurobiol Aging. 1989 Nov-Dec;10(6):717-20. doi: 10.1016/0197-4580(89)90008-0.

DOI:10.1016/0197-4580(89)90008-0

PMID:2534159

Abstract

Differences between Alzheimer and control fibroblast [Ca2+ + Mg2+]-dependent ATPase activity at free Ca2+ concentration considerably higher than physiologic concentrations were observed. At 50 microM free Ca2+, Alzheimer and control fibroblast homogenates exhibited maximum velocity values ranging from 8 to 25 nmoles phosphate released/min/mg protein. Higher free Ca2+ (350 microM) inhibited control fibroblast ATPase activity approximately 77%; whereas, Alzheimer fibroblasts retained greater than 75% starting activity. Although the pathophysiological significance of these findings is at present unclear, these data suggest the Ca2+ pump of Alzheimer fibroblasts behaves differently in the presence of high free Ca2+. Such behavior may be of potential diagnostic value.

摘要

在游离钙离子浓度远高于生理浓度的情况下，观察到阿尔茨海默病成纤维细胞与对照成纤维细胞的[Ca2+ + Mg2+]依赖性ATP酶活性存在差异。在游离钙离子浓度为50微摩尔时，阿尔茨海默病成纤维细胞匀浆和对照成纤维细胞匀浆的最大速度值为每分钟每毫克蛋白质释放8至25纳摩尔磷酸盐。更高的游离钙离子浓度（350微摩尔）使对照成纤维细胞的ATP酶活性受到约77%的抑制；而阿尔茨海默病成纤维细胞保留了超过75%的初始活性。尽管目前这些发现的病理生理学意义尚不清楚，但这些数据表明，在高游离钙离子存在的情况下，阿尔茨海默病成纤维细胞的钙离子泵表现不同。这种行为可能具有潜在的诊断价值。