Dhamole Pradip B, Nair Rashmi R, D'Souza Stanislaus F, Pandit Aniruddha B, Lele S S
Department of Chemical Engineering, Birla Institute of Technology & Science, Pilani, Hyderabad Campus, Hyderabad, 500078, India,
Appl Biochem Biotechnol. 2015 Jan;175(2):748-56. doi: 10.1007/s12010-014-1317-0. Epub 2014 Oct 24.
This work investigates the performance of acclimatized biomass for denitrification of high strength nitrate waste (10,000 mg/L NO3) from a nuclear industry in a continuous laboratory scale (32 L) and pilot scale reactor (330 L) operated over a period of 4 and 5 months, respectively. Effect of substrate fluctuations (mainly C/NO3-N) on denitrification was studied in a laboratory scale reactor. Incomplete denitrification (95-96 %) was observed at low C/NO3-N (≤2), whereas at high C/NO3-N (≥2.25) led to ammonia formation. Ammonia production increased from 1 to 9 % with an increase in C/NO3-N from 2.25 to 6. Complete denitrification and no ammonia formation were observed at an optimum C/NO3-N of 2.0. Microbiological studies showed decrease in denitrifiers and increase in nitrite-oxidizing bacteria and ammonia-oxidizing bacteria at high C/NO3-N (≥2.25). Pilot scale studies were carried out with optimum C/NO3-N, and sustainability of the process was checked on the pilot scale for 5 months.
本研究在连续实验室规模（32升）和中试规模反应器（330升）中，分别运行4个月和5个月，考察了驯化生物质对核工业高强度硝酸盐废水（10,000毫克/升NO₃）进行反硝化的性能。在实验室规模反应器中研究了底物波动（主要是C/NO₃-N）对反硝化的影响。在低C/NO₃-N（≤2）时观察到不完全反硝化（95 - 96%），而在高C/NO₃-N（≥2.25）时导致氨的形成。随着C/NO₃-N从2.25增加到6，氨的产量从1%增加到9%。在最佳C/NO₃-N为2.0时观察到完全反硝化且无氨形成。微生物学研究表明，在高C/NO₃-N（≥2.25）时，反硝化菌减少，亚硝酸盐氧化菌和氨氧化菌增加。在最佳C/NO₃-N条件下进行了中试规模研究，并在中试规模上检查了该过程的可持续性，为期5个月。
