Tao Hui-Kang, Tang Qin, Dai Jin-Jin, Li Yuan-Yuan, Hei Ming-Yan
Department of Pediatrics, Third Xiangya Hospital of Central South University, Changsha 410013, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2014 Oct;16(10):1045-50.
To observe the long-term changes in anxiety-like behavior and tyrosine hydroxylase (TH) expression in the substantia nigra (SN) after hypoxic-ischemic brain damage (HIBD) in a neonatal rat model and to further explore the relationship between dopamine (DA) level and long-term anxiety-like behavior using the DA receptor (DAR) antagonist.
Seven-day-old (P7) neonatal Sprague-Dawley (SD) rats were randomized into normal control, sham-operated, HIBD and HIBD+DAR antagonist groups. HIBD model was prepared by ligating the right common carotid artery and 8% hypoxia exposure. The rats in the sham-operated group were sham-operated and were not subjected to right common carotid artery ligation and hypoxia exposure. The DAR antagonist was injected intraperitoneally before and after inducing HIBD. The same amount of normal saline was given to the other three groups as a control. Anxiety-like behavior was evaluated by elevated plus maze test, and TH expression in the SN was measured by immunohistochemistry on P14, P21, and P28.
On P21 and P28, the time spent in the open arms and the percentage of open arms entries in the HIBD group were significantly increased compared with those in the normal control, sham-operated and HIBD+DAR antagonist groups (P<0.05); in addition, the HIBD+DAR antagonist group showed a significantly longer time spent in the open arms than the normal control group (P<0.05). On P14, P21, and P28, TH expression in the HIBD and HIBD+DAR antagonist groups was significantly lower than that in the normal control and sham-operated groups, and TH level in the HIBD group was significantly lower than that in the HIBD+DAR antagonist group (P<0.05).
DAR antagonist allows the restoration of anxiety-like behavior and alleviates the damage to dopaminergic neurons in SD rats after HIBD.
观察新生大鼠缺氧缺血性脑损伤（HIBD）模型中，黑质（SN）中焦虑样行为及酪氨酸羟化酶（TH）表达的长期变化，并使用多巴胺（DA）受体（DAR）拮抗剂进一步探讨DA水平与长期焦虑样行为之间的关系。
将7日龄（P7）的新生Sprague-Dawley（SD）大鼠随机分为正常对照组、假手术组、HIBD组和HIBD+DAR拮抗剂组。通过结扎右侧颈总动脉并暴露于8%的低氧环境制备HIBD模型。假手术组大鼠进行假手术，不进行右侧颈总动脉结扎和低氧暴露。在诱导HIBD前后腹腔注射DAR拮抗剂。其他三组给予等量生理盐水作为对照。采用高架十字迷宫试验评估焦虑样行为，并在P14、P21和P28通过免疫组织化学法检测SN中TH的表达。
在P21和P28时，HIBD组在开放臂的停留时间和进入开放臂的百分比与正常对照组、假手术组和HIBD+DAR拮抗剂组相比显著增加（P<0.05）；此外，HIBD+DAR拮抗剂组在开放臂的停留时间明显长于正常对照组（P<0.05）。在P
