Laytragoon-Lewin Nongnit, Jönson Fredrik, Lundgren Jan, Rutqvist Lars Erik, Wikby Anders, Löfgren Sture, Lewin Freddi
Department of Laboratory Medicine, Ryhov Hospital, SE-55185, Jönköping, Sweden,
Med Oncol. 2014 Dec;31(12):290. doi: 10.1007/s12032-014-0290-3. Epub 2014 Oct 25.
Long-term survival of H&N cancer patients has not improved significantly over the last 30 years. The possibility of using circulating blood cell phenotypes as a prognostic biomarker of H&N cancer patient was investigated in this study. Pre-treatment, circulating T lymphocyte subpopulations as well as the survival time of the patients in question were studied. Upregulated CD4+ perforin+ and CD8+ CD95+ but downregulated CD4+ CD28+ (p < 0.001) were detected in H&N cancer patients. With 3 years of follow-up time, an increase in the frequency of the pre-treatment, circulating CD4+ perforin+ cells and CD8+ perforin+ cells was showed to have reverse effects on the survival time in H&N cancer patients (p < 0.01). Detection of perforin+ frequency in CD4+ and CD8+ lymphocyte by FACS is fast, simple and cost-effective. A potential role of perforin expression in CD4+ and CD8+ cells as a prognostic biomarker for H&N cancer patient in the clinical setting was suggested.
在过去30年里，头颈癌患者的长期生存率并未显著提高。本研究探讨了将循环血细胞表型用作头颈癌患者预后生物标志物的可能性。研究了预处理时循环T淋巴细胞亚群以及相关患者的生存时间。在头颈癌患者中检测到CD4+穿孔素+和CD8+ CD95+上调，但CD4+ CD28+下调（p<0.001）。经过3年的随访时间，结果显示预处理时循环CD4+穿孔素+细胞和CD8+穿孔素+细胞频率的增加对头颈癌患者的生存时间有相反影响（p<0.01）。通过流式细胞术检测CD4+和CD8+淋巴细胞中穿孔素+频率快速、简单且具有成本效益。提示了穿孔素在CD4+和CD8+细胞中的表达在临床环境中作为头颈癌患者预后生物标志物的潜在作用。
