[The immune cytochemical evaluation of reaction of phytohemagglutinin stimulation of lymphocytes with monoclonal antibodies Ki-67].

The article proposes new (immune cytochemical) mode of evaluation of reaction of blast-cell transformation of human lymphocytes under their stimulation with phytohemagglutinin instead of standard radiometric method using 3H-thymidine as a marker. The reaction was assessed by means of luminescent microscope under indirect immunofluorescence using nuclear antigen Ki-67 as a marker of proliferating cells. The Ki-67 was detected using monoclonal antibodies Ki-67. The functional capacity of lymphocytes was evaluated by amount of visualized (luminescent) Ki-67 positive cells appearing after three day stimulation of proliferation with phytohemagglutinin. The comparison was made between immune cytochemical (with Ki-67 marker) and radiometric (with marking by 3H-thymidine) methods of phytohemagglutinin stimulation of lymphocytes. The article presents substantiation of advantage of immune cytochemical method of evaluation of reaction of blast-cell transformation of human lymphocytes as compared with radiometric method concerning possibility of its broader implementation in laboratory practice.