Krzyzowska-Gruca S, Gruca S, Zborek A, Filipowska M
Department of Tumor Biology, M. Sklodowska-Curie Memorial Institute, Gliwice, Poland.
Histochemistry. 1988;88(3-6):563-9. doi: 10.1007/BF00570325.
We studied the formation of interchromatin granules (IGs) in phytohemagglutinin (PHA)-stimulated lymphocytes. The bismuth staining method was used for the visualization of IGs, and we also applied high-resolution autoradiography after incubating cells in the presence of 3H-leucine during different stages of lymphocyte activation. The disaggregation of chromatin and the enlargement of interchromatinic areas in stimulated lymphocytes were found to be accompanied by an increase in the number of IGs, and it was shown that IGs were formed during all of the investigated stages of lymphocyte stimulation.
我们研究了植物血凝素（PHA）刺激的淋巴细胞中染色质间颗粒（IGs）的形成。采用铋染色法观察IGs，并且在淋巴细胞激活的不同阶段，于3H-亮氨酸存在的情况下孵育细胞后，我们还应用了高分辨率放射自显影术。结果发现，刺激淋巴细胞中染色质的解聚和染色质间区域的扩大伴随着IGs数量的增加，并且表明在淋巴细胞刺激的所有研究阶段均形成了IGs。
