Ryndock Eric J, Biryukov Jennifer, Meyers Craig
Department of Microbiology and Immunology H107, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA.
Methods Mol Biol. 2015;1249:39-52. doi: 10.1007/978-1-4939-2013-6_3.
Human papillomaviruses (HPV) are the major factor in causing cervical cancer as well as being implicated in causing oral and anal cancers. The life cycle of HPV is tied to the epithelial differentiation system, as only native virus can be produced in stratified human skin. Initially, HPV research was only possible utilizing recombinant systems in monolayer culture. With new cell culture technology, systems using differentiated skin have allowed HPV to be studied in its native environment. Here, we describe current research studying native virions in differentiated skin including viral assembly, maturation, capsid protein interactions, and L2 cross-neutralizing epitopes. In doing so, we hope to show how differentiating skin systems have increased our knowledge of HPV biology and identify gaps in our knowledge about this important virus.
人乳头瘤病毒（HPV）是导致宫颈癌的主要因素，同时也与口腔癌和肛门癌的发生有关。HPV的生命周期与上皮分化系统相关，因为只有在分层的人类皮肤中才能产生天然病毒。最初，HPV研究只能利用单层培养中的重组系统进行。随着新的细胞培养技术的出现，使用分化皮肤的系统使HPV能够在其天然环境中进行研究。在此，我们描述了当前在分化皮肤中研究天然病毒粒子的研究，包括病毒组装、成熟、衣壳蛋白相互作用以及L2交叉中和表位。通过这样做，我们希望展示分化皮肤系统如何增加了我们对HPV生物学的了解，并找出我们对这种重要病毒认识上的差距。
