Liu Qiu-Ning, Lin Kun-Zhang, Yang Lin-Nan, Dai Li-Shang, Wang Lei, Sun Yu, Qian Cen, Wei Guo-Qing, Liu Dong-Ran, Zhu Bao-Jian, Liu Chao-Liang
College of Life Sciences, Anhui Agricultural University, Hefei, P. R.China.
Arch Insect Biochem Physiol. 2015 Mar;88(3):155-67. doi: 10.1002/arch.21210. Epub 2014 Oct 27.
Apolipophorin-III (ApoLp-III) acts in lipid transport, lipoprotein metabolism, and innate immunity in insects. In this study, an ApoLp-III gene of Antheraea pernyi pupae (Ap-ApoLp-III) was isolated and characterized. The full-length cDNA of Ap-ApoLp-III is 687 bp, including a 5'-untranslated region (UTR) of 40 bp, 3'-UTR of 86 bp and an open reading frame of 561 bp encoding a polypeptide of 186 amino acids that contains an Apolipophorin-III precursor domain (PF07464). The deduced Ap-apoLp-III protein sequence has 68, 59, and 23% identity with its orthologs of Manduca sexta, Bombyx mori, and Aedes aegypti, respectively. Phylogenetic analysis showed that the Ap-apoLp-III was close to that of Bombycoidea. qPCR analysis revealed that Ap-ApoLp-III expressed during the four developmental stages and in integument, fat body, and ovaries. After six types of microorganism infections, expression levels of the Ap-ApoLp-III gene were upregulated significantly at different time points compared with control. RNA interference (RNAi) of Ap-ApoLp-III showed that the expression of Ap-ApoLp-III was significantly downregulated using qPCR after injection of E. coli. We infer that the Ap-ApoLp-III gene acts in the innate immunity of A. pernyi.
载脂蛋白-III（ApoLp-III）在昆虫的脂质运输、脂蛋白代谢和先天免疫中发挥作用。在本研究中，分离并鉴定了柞蚕蛹的ApoLp-III基因（Ap-ApoLp-III）。Ap-ApoLp-III的全长cDNA为687 bp，包括40 bp的5'-非翻译区（UTR）、86 bp的3'-UTR和一个561 bp的开放阅读框，编码一个含有载脂蛋白-III前体结构域（PF07464）的186个氨基酸的多肽。推导的Ap-apoLp-III蛋白序列与其在烟草天蛾、家蚕和埃及伊蚊中的直系同源物分别具有68%、59%和23%的同一性。系统发育分析表明，Ap-apoLp-III与蚕蛾总科的亲缘关系较近。qPCR分析显示，Ap-ApoLp-III在四个发育阶段以及体壁、脂肪体和卵巢中均有表达。在六种微生物感染后，与对照相比，Ap-ApoLp-III基因的表达水平在不同时间点均显著上调。对Ap-ApoLp-III进行RNA干扰（RNAi）表明，注射大肠杆菌后，使用qPCR检测发现Ap-ApoLp-III的表达显著下调。我们推断Ap-ApoLp-III基因在柞蚕的先天免疫中发挥作用。
