Kamada A
J Osaka Dent Univ. 1989 Oct;23(2):63-75.
The effect of streptozotocin-induced diabetes on the structure of heparan sulfate (HS) prepared from rat kidney glycosaminoglycans (GAG) was evaluated. GAG were isolated and purified from the kidneys of diabetic and age-matched control rats by standard procedures. HS was prepared from GAG by digestion with chondroitinase ABC and precipitation with cetylpyridinium chloride. The tissue dry weight of diabetic kidneys was greater than that of the controls. The amounts of protein and DNA per tissue dry weight were decreased in the diabetic group, while GAG and hydroxyproline remained unchanged. The above information indicates that the extracellular components are increased in diabetes. There was no significant difference in the amount of HS to tissue dry weight between the diabetic and control groups. When the molecular weight of the HS from both groups was compared by Sephacryl S-300 HR column chromatography, the HS peak for the diabetic kidney indicated a slightly higher molecular weight and the base of the peak was broader than that for the controls. A reduction in N-sulfate residues was observed in Sephadex G-50 profiles after nitrous acid degradation of the HS. The ratio of glucuronic acid to its epimer, iduronic acid, in diabetic kidney HS was slightly lower than that in the controls. This indicates that diabetes may influence the carbohydrate chain structure of the HS in the kidney. Quantitative and qualitative changes in the kidney HS may contribute to the symptoms associated with diabetic nephropathy.
评估链脲佐菌素诱导的糖尿病对从大鼠肾脏糖胺聚糖（GAG）制备的硫酸乙酰肝素（HS）结构的影响。通过标准程序从糖尿病大鼠和年龄匹配的对照大鼠的肾脏中分离并纯化GAG。通过用软骨素酶ABC消化并用十六烷基吡啶氯化物沉淀从GAG制备HS。糖尿病肾脏的组织干重大于对照组。糖尿病组每组织干重的蛋白质和DNA量减少，而GAG和羟脯氨酸保持不变。上述信息表明糖尿病中细胞外成分增加。糖尿病组和对照组之间HS与组织干重的量没有显著差异。当通过Sephacryl S-300 HR柱色谱比较两组HS的分子量时，糖尿病肾脏的HS峰表明分子量略高，且峰底比对照组宽。在HS经亚硝酸降解后的Sephadex G-50图谱中观察到N-硫酸酯残基减少。糖尿病肾脏HS中葡萄糖醛酸与其差向异构体艾杜糖醛酸的比例略低于对照组。这表明糖尿病可能影响肾脏中HS的碳水化合物链结构。肾脏HS的定量和定性变化可能导致与糖尿病肾病相关的症状。
