Wielgus-Serafińska E, Kamiński M, Nowaczyk-Dura G
Pol J Occup Med. 1989;2(1):23-31.
The aim of this study was to determine activities of selected enzymes, i.e. Mg(++)-stimulated ATP-ase, acid phosphatase (AcP), esterase and succinate dehydrogenase (SDH) in rat liver after intragastric administration of cadmium chloride. The investigations were performed on forty male rats, Wistar strain, which made up one control group and two experimental groups. The rats of one experimental group were given a single dose of cadmium chloride solution of 88 mg CdCl2 per kg body weight (Group I), whereas the other experimental group received a double dose, i.e. 176 mg CdCl2 per kg body weight (Group II). The activities of enzymatic reactions were determined by means of analysis of surface optical density using the image computer analyzer, whereas the degree of mental accumulation was estimated with atom absorption spectrophotometry. Following cadmium chloride administration, the activities of SDH and esterase decreased in both experimental groups compared to controls. The decrease in both enzymatic activities was statistically significant. Similarly, activities of ACP and Mg(++)-stimulated ATP-ase decreased below control level in the rats of the Group I, which were given lower dose of CdCl2. This change was not statistically significant. Higher dose of CdCl2 (Group II) caused a statistically significant increase in acid phosphatase activity and only a small increase (bearing no statistical significance) in Mg(++)-stimulated ATP-ase activity. In addition, it was found that the accumulation of cadmium in both the liver and the kidneys is dose-related.
本研究的目的是测定大鼠经胃内给予氯化镉后，所选酶即镁离子刺激的ATP酶、酸性磷酸酶（AcP）、酯酶和琥珀酸脱氢酶（SDH）的活性。研究对40只雄性Wistar大鼠进行，分为一个对照组和两个实验组。一个实验组的大鼠给予单剂量每千克体重88毫克氯化镉溶液（I组），而另一个实验组接受双剂量，即每千克体重176毫克氯化镉（II组）。酶促反应的活性通过使用图像计算机分析仪分析表面光密度来测定，而金属积累程度则用原子吸收分光光度法估算。给予氯化镉后，与对照组相比，两个实验组中SDH和酯酶的活性均降低。这两种酶活性的降低具有统计学意义。同样，给予较低剂量氯化镉的I组大鼠中，ACP和镁离子刺激的ATP酶活性降至对照水平以下。这种变化无统计学意义。较高剂量的氯化镉（II组）导致酸性磷酸酶活性有统计学意义的增加，而镁离子刺激的ATP酶活性仅略有增加（无统计学意义）。此外，还发现肝脏和肾脏中镉的积累与剂量相关。
