Harada Kazuo
Department of Life Sciences, Tokyo Gakugei University, 4-1-1 Nukuikita-machi, Koganei, Tokyo, 184-8501, Japan,
Methods Mol Biol. 2015;1240:49-56. doi: 10.1007/978-1-4939-1896-6_4.
RNA-protein interactions play important roles in gene regulation, functional RNA-protein complexes such as the ribosome, and in viral replication. Molecules that regulate specific RNA-protein interactions may be used to dissect biological processes, and to establish the validity of targeting an RNA-protein interaction. There are many examples of biological regulation by antisense RNA stem-loops that form loop-loop and loop-linear RNA-RNA interactions. Here, a bacterial reporter system for the identification of RNA stem-loops that inhibit the formation of RNA-protein complexes through RNA-RNA interactions is described.
RNA-蛋白质相互作用在基因调控、诸如核糖体等功能性RNA-蛋白质复合物以及病毒复制过程中发挥着重要作用。调控特定RNA-蛋白质相互作用的分子可用于剖析生物学过程，并确定靶向RNA-蛋白质相互作用的有效性。通过形成环-环和环-线性RNA-RNA相互作用的反义RNA茎环进行生物调控的例子有很多。在此，描述了一种用于鉴定通过RNA-RNA相互作用抑制RNA-蛋白质复合物形成的RNA茎环的细菌报告系统。
