Anupam R, Zhou S, Hines J V
Department of Chemistry and Biochemistry, Ohio University, Clippinger Laboratory, 1 Ohio Universitys, Athens, OH, 45701, USA.
Methods Mol Biol. 2015;1240:135-42. doi: 10.1007/978-1-4939-1896-6_10.
Changes in electrophoretic mobility upon complex formation with RNA can be used to probe structure-function relationships that are critical for complex formation. Here, we describe the application of this technique to monitor tRNA binding to the T box riboswitch antiterminator RNA.
与RNA形成复合物时电泳迁移率的变化可用于探究对复合物形成至关重要的结构-功能关系。在此，我们描述了该技术在监测tRNA与T盒核糖开关抗终止子RNA结合方面的应用。
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