Baydemir Gözde
a Division of Biochemistry, Department of Chemistry , Aksaray University , Aksaray , Turkey.
Artif Cells Nanomed Biotechnol. 2016;44(2):610-7. doi: 10.3109/21691401.2014.975236. Epub 2014 Oct 29.
Chondroitin sulfate (Cs)-imprinted poly(hydroxyethyl methacrylate)-based macroporous cryogels (CsMIP) were prepared for selective recognition of Cs from an aqueous solution. The selective binding sites for Cs were maintained using vinyl imidazole-Cu(2+) functional groups, during the precomplexation step in the polymerization procedure. Newly synthesized CsMIP cryogel columns were characterized. The separation of Cs from aqueous solutions was studied, both in the continuous system and in the fast protein liquid chromatography (FPLC) system. According to the FPLC studies, the Rs value obtained was 14.72, which shows that the CsMIP cryogel column can successfully separate Cs from aqueous solutions of Cs in the presence of competitor molecules.
制备了基于硫酸软骨素（Cs）印迹的聚甲基丙烯酸羟乙酯大孔冷冻凝胶（CsMIP），用于从水溶液中选择性识别Cs。在聚合过程的预络合步骤中，使用乙烯基咪唑-Cu(2+)官能团维持Cs的选择性结合位点。对新合成的CsMIP冷冻凝胶柱进行了表征。研究了在连续系统和快速蛋白质液相色谱（FPLC）系统中从水溶液中分离Cs的情况。根据FPLC研究，获得的Rs值为14.72，这表明CsMIP冷冻凝胶柱能够在存在竞争分子的情况下成功地从Cs水溶液中分离出Cs。
