Guelrud M, Mendoza S, Gelrud D, Essenfeld E
G E N. 1989 Oct-Dec;43(4):279-82.
Campylobacter pylori has been implicated in the pathogenesis of peptic ulcer, non-ulcer dyspepsia and chronic gastritis. C. pylori produces large amounts of urease which has been used as a biochemical support to identify the microorganism. In this study, we described the use of a rapid urease test (CLOtest) to detect C. pylori. In 46 consecutive patients, biopsy of fundus and antrum were obtained for histology and CLOtest. All specimens showed chronic gastritis. In 22 patients (48%) C. pylori was identified either by histology or CLOtest. In 18 patients (39%) CLOtest was positive and in 16 patients (35%) histology was positive. In 12 patients (26%) C. pylori was identified by both tests. There was no difference in relation to the presence of C. pylori in fundus and antrum. However, in 2 patients (4%) C. pylori was positive in fundus and negative in antrum. In 9 patients (20%) C. pylori was positive in antrum and negative in fundus. CLOtest is a simple, sensitive and rapid test that enables the endoscopist to diagnose C. pylori infection in the endoscopy room.
幽门螺杆菌与消化性溃疡、非溃疡性消化不良和慢性胃炎的发病机制有关。幽门螺杆菌产生大量脲酶，该酶已被用作鉴定该微生物的生化依据。在本研究中，我们描述了使用快速脲酶试验（CLOtest）检测幽门螺杆菌。在连续46例患者中，获取胃底和胃窦活检组织用于组织学检查和CLOtest检测。所有标本均显示为慢性胃炎。22例患者（48%）通过组织学检查或CLOtest检测鉴定出幽门螺杆菌。18例患者（39%）CLOtest检测呈阳性，16例患者（35%）组织学检查呈阳性。12例患者（26%）两种检测均鉴定出幽门螺杆菌。胃底和胃窦中幽门螺杆菌的存在情况无差异。然而，2例患者（4%）胃底幽门螺杆菌呈阳性而胃窦呈阴性。9例患者（20%）胃窦幽门螺杆菌呈阳性而胃底呈阴性。CLOtest是一种简单、灵敏且快速的检测方法，可使内镜医师在内镜检查室诊断幽门螺杆菌感染。
