Perry E S, Miller W R, Lindsay S
Center for Biodiversity, Unity College, Unity, Maine, 04988, U.S.A.
J Microsc. 2015 Feb;257(2):117-22. doi: 10.1111/jmi.12190. Epub 2014 Oct 29.
The use of epifluorescence microscopy coupled with ultraviolet (UV) autofluorescence is suggested as a means to view and interpret tardigrade structures. Endogenous fluorochromes are a known component of tardigrade cuticle, claws and bucco-pharyngeal apparatus. By imaging the autofluorescence from tardigrades, it is possible to document these structures in detail, including the subdivisions and boundaries of echiniscid (heterotardigrade) plates and the nature and spatial relationships of the texture (pores, granules, papillae and tubercles) on the various plates. This allows the determination of taxonomic features not easily seen with other microscopic techniques.
建议使用落射荧光显微镜结合紫外（UV）自发荧光来观察和解释缓步动物的结构。内源性荧光色素是缓步动物角质层、爪子和口咽器官的已知组成部分。通过对缓步动物的自发荧光进行成像，可以详细记录这些结构，包括棘影目（异缓步动物）板的细分和边界以及不同板上纹理（孔、颗粒、乳头和瘤）的性质和空间关系。这使得能够确定其他显微镜技术不易看到的分类特征。
